Continuous production of carotenoids from Dunaliella salina

D.M.M. Kleinegris, M.G.J. Janssen, W.A. Brandenburg, R.H. Wijffels

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

During the in situ extraction of ß-carotene from Dunaliella salina, the causal relationship between carotenoid extraction and cell death indicated that cell growth and cell death should be at equilibrium for a continuous in situ extraction process. In a flat-panel photobioreactor that was operated as a turbidostat cell numbers of stressed cells were kept constant while attaining a continuous well-defined light-stress. In this way it was possible to study the balance between cell growth and cell death and determine whether both could be increased to reach higher volumetric productivities of carotenoids. In the two-phase system a volumetric productivity of 8.3 mg ß-carotene LRV-1 d-1 was obtained. In situ extraction contributed only partly to this productivity. The major part came from net production of carotenoid-rich biomass, due to a high growth rate of the cells and subsequent dilution of the reactor. To reach equilibrium between cell growth and cell death, sparging rates of dodecane could have been increased. However, already at the applied sparging rate of 286 Ldod LRV-1 min-1 emulsion formation of the dodecane in the aqueous phase appeared. In a turbidostat without in situ extraction a volumetric productivity of 13.5 mg ß-carotene LRV-1 d-1 was reached, solely based on the continuous production of carotenoid-rich biomass.
Original languageEnglish
Pages (from-to)253-259
JournalEnzyme and Microbial Technology
Volume48
Issue number3
DOIs
Publication statusPublished - 2011

Fingerprint

Carotenoids
Cell death
Cell growth
Cell Death
Productivity
Growth
Biomass
Photobioreactors
Salinum
Emulsions
Dilution
Cell Count
Light
Mortality

Keywords

  • beta-carotene
  • 2-phase bioreactors
  • accumulation
  • chlorophyceae
  • extraction
  • growth
  • light
  • rich

Cite this

Kleinegris, D.M.M. ; Janssen, M.G.J. ; Brandenburg, W.A. ; Wijffels, R.H. / Continuous production of carotenoids from Dunaliella salina. In: Enzyme and Microbial Technology. 2011 ; Vol. 48, No. 3. pp. 253-259.
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Continuous production of carotenoids from Dunaliella salina. / Kleinegris, D.M.M.; Janssen, M.G.J.; Brandenburg, W.A.; Wijffels, R.H.

In: Enzyme and Microbial Technology, Vol. 48, No. 3, 2011, p. 253-259.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Janssen, M.G.J.

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AU - Wijffels, R.H.

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