Continuous lactose fermentation by Clostridium acetobutylicum - Assessment of solventogenic kinetics

Alessandra Procentese, Francesca Raganati, Giuseppe Olivieri*, Maria Elena Russo, Piero Salatino, Antonio Marzocchella

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)


This work reports the results of a series of tests on the specific butanol production rate by Clostridium acetobutylicum continuous cultures. The tests were carried out using lactose as carbon source to mimic cheese-whey. A continuous stirred tank reactor equipped with a microfiltration unit was used. The dilution rate (D) ranged between 0.02 and 0.15h-1 and the ratio R of the permeate stream rate to the stream fed to the reactor ranged between 14% and 95%. For each set of D and R values, the continuous cultures were characterized in terms of concentration of cells, acids and solvents. Results were processed to assess the concentration of acidogenic cells, solventogenic cells, spores and the specific butanol production rate. The max butanol productivity was 0.5gL-1h-1 at D=0.1h-1 and R=95%. The butanol productivity referred to solventogenic cells was expressed as a function of concentration of lactose, acids and butanol.

Original languageEnglish
Pages (from-to)330-337
JournalBioresource Technology
Publication statusPublished - 2015


  • Butanol
  • Clostridium acetobutylicum
  • Kinetic model
  • Microfiltration
  • Solventogenesis

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