Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

M.L. Mohedano, T. Garcia-Cayuela, A. Perez-Ramos, R.A. Gaiser, T. Requena, P. Lopez

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Lactobacilli are widespread in natural environments and are increasingly being investigated as potential health modulators. In this study, we have adapted the broad-host-range vector pNZ8048 to express the mCherry protein (pRCR) to expand the usage of the mCherry protein for analysis of gene expression in Lactobacillus. This vector is also able to replicate in Streptococcus pneumoniae and Escherichia coli. The usage of pRCR as a promoter probe was validated in Lactobacillus acidophilus by characterizing the regulation of lactacin B expression. The results show that the regulation is exerted at the transcriptional level, with lbaB gene expression being specifically induced by co-culture of the L. acidophilus bacteriocin producer and the S. thermophilus STY-31 inducer bacterium.
Original languageEnglish
Pages (from-to)247-253
JournalJournal of Industrial Microbiology and Biotechnology
Volume42
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • lactic-acid bacteria
  • controlled gene-expression
  • streptococcus-pneumoniae
  • lactococcus-lactis
  • plasmid
  • cloning

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