Constraints on the Biological Source(s) of the Orphan Branched Tetraether Membrane Lipids

J.W.H. Weijers; E. Panoto; J. van Bleiswijk; S. Schouten; M. Balk; A.J.M. Stams; W.I.C. Rijpstra; J.S. Sinninghe Damsté

doi:10.1080/01490450902937293

Constraints on the Biological Source(s) of the Orphan Branched Tetraether Membrane Lipids

J.W.H. Weijers, E. Panoto, J. van Bleiswijk, S. Schouten, M. Balk, A.J.M. Stams, W.I.C. Rijpstra, J.S. Sinninghe Damsté

Research output: Contribution to journal › Article › Academic › peer-review

212 Citations (Scopus)

Abstract

A soil profile from the Saxns Mosse peat bog, Sweden, has been analysed for glycerol dialkyl glycerol tetraether (GDGT) membrane lipids and 16S rRNA genes in order to constrain the source of the yet 'orphan,' but supposedly bacterial, branched GDGTs. Branched GDGT lipids dominate over archaeal membrane lipids. The Acidobacteria comprise the dominant bacterial group, accounting for the majority of total Bacteria, and are generally more abundant than methanogenic archaea. Analysed acidobacterial strains did not contain branched GDGT lipids. Thus, the source organism must likely be searched for in other acidobacterial phyla or in another abundant group within the remaining bacteria

Original language	English
Pages (from-to)	402-414
Journal	Geomicrobiology Journal
Volume	26
Issue number	6
DOIs	https://doi.org/10.1080/01490450902937293
Publication status	Published - 2009

Keywords

16s ribosomal-rna
gradient gel-electrophoresis
southwestern united-states
polymerase-chain-reaction
ombrotrophic peat bog
nov sp-nov
acid-tolerant
thermoanaerobacter-ethanolicus
acidobacterium-capsulatum
phylogenetic analysis

Access to Document

10.1080/01490450902937293

Cite this

@article{176843daec0e44a6a1f64a8d1d6c7823,

title = "Constraints on the Biological Source(s) of the Orphan Branched Tetraether Membrane Lipids",

abstract = "A soil profile from the Saxns Mosse peat bog, Sweden, has been analysed for glycerol dialkyl glycerol tetraether (GDGT) membrane lipids and 16S rRNA genes in order to constrain the source of the yet 'orphan,' but supposedly bacterial, branched GDGTs. Branched GDGT lipids dominate over archaeal membrane lipids. The Acidobacteria comprise the dominant bacterial group, accounting for the majority of total Bacteria, and are generally more abundant than methanogenic archaea. Analysed acidobacterial strains did not contain branched GDGT lipids. Thus, the source organism must likely be searched for in other acidobacterial phyla or in another abundant group within the remaining bacteria",

keywords = "16s ribosomal-rna, gradient gel-electrophoresis, southwestern united-states, polymerase-chain-reaction, ombrotrophic peat bog, nov sp-nov, acid-tolerant, thermoanaerobacter-ethanolicus, acidobacterium-capsulatum, phylogenetic analysis",

author = "J.W.H. Weijers and E. Panoto and \{van Bleiswijk\}, J. and S. Schouten and M. Balk and A.J.M. Stams and W.I.C. Rijpstra and \{Sinninghe Damst{\'e}\}, J.S.",

year = "2009",

doi = "10.1080/01490450902937293",

language = "English",

volume = "26",

pages = "402--414",

journal = "Geomicrobiology Journal",

issn = "0149-0451",

publisher = "Taylor \& Francis",

number = "6",

}

TY - JOUR

T1 - Constraints on the Biological Source(s) of the Orphan Branched Tetraether Membrane Lipids

AU - Weijers, J.W.H.

AU - Panoto, E.

AU - van Bleiswijk, J.

AU - Schouten, S.

AU - Balk, M.

AU - Stams, A.J.M.

AU - Rijpstra, W.I.C.

AU - Sinninghe Damsté, J.S.

PY - 2009

Y1 - 2009

N2 - A soil profile from the Saxns Mosse peat bog, Sweden, has been analysed for glycerol dialkyl glycerol tetraether (GDGT) membrane lipids and 16S rRNA genes in order to constrain the source of the yet 'orphan,' but supposedly bacterial, branched GDGTs. Branched GDGT lipids dominate over archaeal membrane lipids. The Acidobacteria comprise the dominant bacterial group, accounting for the majority of total Bacteria, and are generally more abundant than methanogenic archaea. Analysed acidobacterial strains did not contain branched GDGT lipids. Thus, the source organism must likely be searched for in other acidobacterial phyla or in another abundant group within the remaining bacteria

AB - A soil profile from the Saxns Mosse peat bog, Sweden, has been analysed for glycerol dialkyl glycerol tetraether (GDGT) membrane lipids and 16S rRNA genes in order to constrain the source of the yet 'orphan,' but supposedly bacterial, branched GDGTs. Branched GDGT lipids dominate over archaeal membrane lipids. The Acidobacteria comprise the dominant bacterial group, accounting for the majority of total Bacteria, and are generally more abundant than methanogenic archaea. Analysed acidobacterial strains did not contain branched GDGT lipids. Thus, the source organism must likely be searched for in other acidobacterial phyla or in another abundant group within the remaining bacteria

KW - 16s ribosomal-rna

KW - gradient gel-electrophoresis

KW - southwestern united-states

KW - polymerase-chain-reaction

KW - ombrotrophic peat bog

KW - nov sp-nov

KW - acid-tolerant

KW - thermoanaerobacter-ethanolicus

KW - acidobacterium-capsulatum

KW - phylogenetic analysis

U2 - 10.1080/01490450902937293

DO - 10.1080/01490450902937293

M3 - Article

SN - 0149-0451

VL - 26

SP - 402

EP - 414

JO - Geomicrobiology Journal

JF - Geomicrobiology Journal

IS - 6

ER -

Constraints on the Biological Source(s) of the Orphan Branched Tetraether Membrane Lipids

Abstract

Keywords

Access to Document

Fingerprint

Cite this