Confirmation and 3D profiling of anabolic steroid esters in injection sites using imaging desorption electrospray ionisation (DESI) mass spectrometry

E. de Rijke, H. Hooijerink, S.S. Sterk, M.W.F. Nielen

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Abstract

In this study, desorption electrospray ionization (DESI) linear ion trap tandem mass spectrometry (MSn) was applied for the confirmation and 3D profiling of anabolic steroid esters in an injection site of bovine muscle. The spatial resolution of the DESI-MSn was demonstrated by scanning hormone esters and marker ink lines drawn at various distances on a microscopic slide at set distances, using an x-scanner with manual y and z adjustment. Tissue slices of bovine muscle injected with a hormone cocktail were analysed. All anabolic steroid esters could be directly detected in the sample and confirmed based on identification points awarded for selected MS/MS transitions according to the performance criteria in Commission Decision 2002/657/EC. Moreover, the injection site could be mapped by 2D and 3D imaging MS, showing a horizontal and vertical distribution through the muscle tissue. This DESI approach offers potential for analysis of injection sites of steroid esters from illegally treated animals; moreover, direct analysis by ambient imaging DESI-MS still allows conventional extraction and analysis of the whole tissue for further confirmatory or contraanalysis afterwards.
Original languageEnglish
Pages (from-to)1012-1019
JournalFood Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Volume30
Issue number6
DOIs
Publication statusPublished - 2013

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Testosterone Congeners
anabolic steroids
Electrospray ionization
Electrospray Ionization Mass Spectrometry
injection site
desorption
ionization
Mass spectrometry
Muscle
Desorption
Esters
esters
image analysis
Tissue
Imaging techniques
Muscles
Injections
hormones
Hormones
muscles

Keywords

  • tissues
  • cocktails
  • brain
  • drugs

Cite this

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title = "Confirmation and 3D profiling of anabolic steroid esters in injection sites using imaging desorption electrospray ionisation (DESI) mass spectrometry",
abstract = "In this study, desorption electrospray ionization (DESI) linear ion trap tandem mass spectrometry (MSn) was applied for the confirmation and 3D profiling of anabolic steroid esters in an injection site of bovine muscle. The spatial resolution of the DESI-MSn was demonstrated by scanning hormone esters and marker ink lines drawn at various distances on a microscopic slide at set distances, using an x-scanner with manual y and z adjustment. Tissue slices of bovine muscle injected with a hormone cocktail were analysed. All anabolic steroid esters could be directly detected in the sample and confirmed based on identification points awarded for selected MS/MS transitions according to the performance criteria in Commission Decision 2002/657/EC. Moreover, the injection site could be mapped by 2D and 3D imaging MS, showing a horizontal and vertical distribution through the muscle tissue. This DESI approach offers potential for analysis of injection sites of steroid esters from illegally treated animals; moreover, direct analysis by ambient imaging DESI-MS still allows conventional extraction and analysis of the whole tissue for further confirmatory or contraanalysis afterwards.",
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author = "{de Rijke}, E. and H. Hooijerink and S.S. Sterk and M.W.F. Nielen",
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T1 - Confirmation and 3D profiling of anabolic steroid esters in injection sites using imaging desorption electrospray ionisation (DESI) mass spectrometry

AU - de Rijke, E.

AU - Hooijerink, H.

AU - Sterk, S.S.

AU - Nielen, M.W.F.

PY - 2013

Y1 - 2013

N2 - In this study, desorption electrospray ionization (DESI) linear ion trap tandem mass spectrometry (MSn) was applied for the confirmation and 3D profiling of anabolic steroid esters in an injection site of bovine muscle. The spatial resolution of the DESI-MSn was demonstrated by scanning hormone esters and marker ink lines drawn at various distances on a microscopic slide at set distances, using an x-scanner with manual y and z adjustment. Tissue slices of bovine muscle injected with a hormone cocktail were analysed. All anabolic steroid esters could be directly detected in the sample and confirmed based on identification points awarded for selected MS/MS transitions according to the performance criteria in Commission Decision 2002/657/EC. Moreover, the injection site could be mapped by 2D and 3D imaging MS, showing a horizontal and vertical distribution through the muscle tissue. This DESI approach offers potential for analysis of injection sites of steroid esters from illegally treated animals; moreover, direct analysis by ambient imaging DESI-MS still allows conventional extraction and analysis of the whole tissue for further confirmatory or contraanalysis afterwards.

AB - In this study, desorption electrospray ionization (DESI) linear ion trap tandem mass spectrometry (MSn) was applied for the confirmation and 3D profiling of anabolic steroid esters in an injection site of bovine muscle. The spatial resolution of the DESI-MSn was demonstrated by scanning hormone esters and marker ink lines drawn at various distances on a microscopic slide at set distances, using an x-scanner with manual y and z adjustment. Tissue slices of bovine muscle injected with a hormone cocktail were analysed. All anabolic steroid esters could be directly detected in the sample and confirmed based on identification points awarded for selected MS/MS transitions according to the performance criteria in Commission Decision 2002/657/EC. Moreover, the injection site could be mapped by 2D and 3D imaging MS, showing a horizontal and vertical distribution through the muscle tissue. This DESI approach offers potential for analysis of injection sites of steroid esters from illegally treated animals; moreover, direct analysis by ambient imaging DESI-MS still allows conventional extraction and analysis of the whole tissue for further confirmatory or contraanalysis afterwards.

KW - tissues

KW - cocktails

KW - brain

KW - drugs

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JO - Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment

JF - Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment

SN - 1944-0049

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ER -