Computational Prediction of Functional MicroRNA–mRNA Interactions

Müşerref D. Saçar Demirci, , Malik Yousef, Jens Allmer

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Proteins have a strong influence on the phenotype and their aberrant expression leads to diseases. MicroRNAs (miRNAs) are short RNA sequences which posttranscriptionally regulate protein expression. This regulation is driven by miRNAs acting as recognition sequences for their target mRNAs within a larger regulatory machinery. A miRNA can have many target mRNAs and an mRNA can be targeted by many miRNAs which makes it difficult to experimentally discover all miRNA–mRNA interactions. Therefore, computational methods have been developed for miRNA detection and miRNA target prediction. An abundance of available computational tools makes selection difficult. Additionally, interactions are not currently the focus of investigation although they more accurately define the regulation than pre-miRNA detection or target prediction could perform alone. We define an interaction including the miRNA source and the mRNA target. We present computational methods allowing the investigation of these interactions as well as how they can be used to extend regulatory pathways. Finally, we present a list of points that should be taken into account when investigating miRNA–mRNA interactions. In the future, this may lead to better understanding of functional interactions which may pave the way for disease marker discovery and design of miRNA-based drugs.
Original languageEnglish
Title of host publicationComputational Biology of Non-Coding RNA
EditorsX. Lai, S.K. Gupta, J. Vera
Place of PublicationNew York
PublisherSpringer
Pages175-196
ISBN (Electronic)9781493989829
ISBN (Print)9781493989812
DOIs
Publication statusPublished - 12 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1912
ISSN (Electronic)1940-6029

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Saçar Demirci, , M. D., Yousef, M., & Allmer, J. (2019). Computational Prediction of Functional MicroRNA–mRNA Interactions. In X. Lai, S. K. Gupta, & J. Vera (Eds.), Computational Biology of Non-Coding RNA (pp. 175-196). (Methods in Molecular Biology; Vol. 1912). New York: Springer. https://doi.org/10.1007/978-1-4939-8982-9_7