Comprehensive two-dimensional liquid chromatography with ultraviolet, evaporative light scattering and mass spectrometric detection of triacylglycerols in corn oil

E.J.C. van der Klift, G. Vivó-Truyols, F.W. Claassen, F.L. van Holthoon, T.A. van Beek

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Abstract

An improved comprehensive two-dimensional (LC × LC) HPLC system for the analysis of triacylglycerols was developed. In the first-dimension, a Ag(I)-coated cation exchanger (250 mm × 2.1 mm, 5 ¿m) was employed with a gradient from 100% MeOH to 6% MeCN in MeOH at 20 ¿L/min. Using a 10-way valve with two switching loops, 1 min sections of the first-dimension were introduced in the second-dimension consisting of a 30 mm × 4.6 mm C18 (1.8 ¿m) column with an isocratic mobile phase of methanol¿methyl tert-butyl ether (70:30) at 3.0 mL/min. As the second-dimension solvent was stronger than the first-dimension solvent, focusing in the second-dimension took place, leading to better separations than in previously reported analyses in which hexane was the main constituent of the first-dimension eluent. Compounds differing by 2 in their partition number were baseline separated in the second-dimension. Detection took place by UV at 210 nm, evaporative light scattering and (+)-atmospheric pressure chemical ionisation-MS with the latter giving the best results. Corn oil was investigated and 44 compounds could be detected: 34 triacylglycerols (TAGs), 8 oxygenated TAGs, and 2 TAGs containing a trans double bond. Data manipulation allowed the construction of contour plots and the automated calculation of the first- and second-dimension retention times and peak areas. Quantitative results are compared with a fatty acid methyl ester analysis, and with literature data.
Original languageEnglish
Pages (from-to)43-55
JournalJournal of Chromatography. A, Including electrophoresis and other separation methods
Volume1178
Issue number1-2
DOIs
Publication statusPublished - 2008

Keywords

  • plant oils
  • argentation chromatography
  • gas-chromatography
  • reversed-phase
  • performance
  • separation
  • column
  • lipids
  • matrix
  • fats

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