Comprehensive phenotyping reveals interactions and functions of Arabidopsis thaliana TCP genes in yield determination

S.W. van Es, Elwin van der Auweraert, Sylvia Rodrigues da Silveira, G.C. Angenent, A.D.J. van Dijk, G.H. Immink

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Members of the Arabidopsis thaliana TCP transcription factor (TF) family affect plant growth and development. We systematically quantified the effect of mutagenizing single or multiple TCP TFs and how altered vegetative growth or branching influences final seed yield. We monitored rosette growth over time andbranching patterns and seed yield characteristics at the end of the lifecycle. Subsequently, an approach was developed to disentangle vegetative growth and to determine possible effects on seed yield. Analysis of growth parameters showed all investigated tcp mutants to be affected in certain growth aspects compared with wild-type plants, highlighting the importance of TCP TFs in plant development. Furthermore, we found evidence that all class II TCPs are involved in axillary branch outgrowth, either as inhibitors (BRANCHED-like genes) or enhancers (JAW- and TCP5-like genes). Comprehensive phenotyping of plants mutant for single or multiple TCP TFs reveals that the proposed opposite functions of class I and class II TCPs in plant growth needs revision and shows complex interactions between closely related TCP genes instead of full geneticredundancy. In various instances, the alterations in vegetative growth or in branching patterns result into negative trade-off effects on seed yield that were missed in previous studies, showing the importance of comprehensive and quantitative phenotyping.
LanguageEnglish
JournalThe Plant Journal
DOIs
Publication statusE-pub ahead of print - 22 Mar 2019

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Arabidopsis
seed yield
Arabidopsis thaliana
vegetative growth
phenotype
Growth
Seeds
Genes
plant development
branching
genes
plant growth
Plant Development
mutants
growth and development
transcription factors
Growth and Development
Transcription Factors

Cite this

@article{73c59f866b6d42b9be1d7390f97f672e,
title = "Comprehensive phenotyping reveals interactions and functions of Arabidopsis thaliana TCP genes in yield determination",
abstract = "Members of the Arabidopsis thaliana TCP transcription factor (TF) family affect plant growth and development. We systematically quantified the effect of mutagenizing single or multiple TCP TFs and how altered vegetative growth or branching influences final seed yield. We monitored rosette growth over time andbranching patterns and seed yield characteristics at the end of the lifecycle. Subsequently, an approach was developed to disentangle vegetative growth and to determine possible effects on seed yield. Analysis of growth parameters showed all investigated tcp mutants to be affected in certain growth aspects compared with wild-type plants, highlighting the importance of TCP TFs in plant development. Furthermore, we found evidence that all class II TCPs are involved in axillary branch outgrowth, either as inhibitors (BRANCHED-like genes) or enhancers (JAW- and TCP5-like genes). Comprehensive phenotyping of plants mutant for single or multiple TCP TFs reveals that the proposed opposite functions of class I and class II TCPs in plant growth needs revision and shows complex interactions between closely related TCP genes instead of full geneticredundancy. In various instances, the alterations in vegetative growth or in branching patterns result into negative trade-off effects on seed yield that were missed in previous studies, showing the importance of comprehensive and quantitative phenotyping.",
author = "{van Es}, S.W. and {van der Auweraert}, Elwin and {Rodrigues da Silveira}, Sylvia and G.C. Angenent and {van Dijk}, A.D.J. and G.H. Immink",
year = "2019",
month = "3",
day = "22",
doi = "10.1111/tpj.14326",
language = "English",
journal = "The Plant Journal",
issn = "0960-7412",
publisher = "Wiley",

}

Comprehensive phenotyping reveals interactions and functions of Arabidopsis thaliana TCP genes in yield determination. / van Es, S.W.; van der Auweraert, Elwin; Rodrigues da Silveira, Sylvia; Angenent, G.C.; van Dijk, A.D.J.; Immink, G.H.

In: The Plant Journal, 22.03.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van Es, S.W.

AU - van der Auweraert, Elwin

AU - Rodrigues da Silveira, Sylvia

AU - Angenent, G.C.

AU - van Dijk, A.D.J.

AU - Immink, G.H.

PY - 2019/3/22

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