Comprehensive interaction map of the Arabidopsis MADS box transcription factors

S. de Folter, R.G.H. Immink, M. Kieffer, L. Parenicová, S.R. Henz, D. Weigel, M. Busscher, K. Kooiker, L. Colombo, M.M. Kater, B. Davis, G.C. Angenent

    Research output: Contribution to journalArticleAcademicpeer-review

    478 Citations (Scopus)

    Abstract

    Interactions between proteins are essential for their functioning and the biological processes they control. The elucidation of interaction maps based on yeast studies is a first step toward the understanding of molecular networks and provides a framework of proteins that possess the capacity and specificity to interact. Here, we present a comprehensive plant protein–protein interactome map of nearly all members of the Arabidopsis thaliana MADS box transcription factor family. A matrix-based yeast two-hybrid screen of >100 members of this family revealed a collection of specific heterodimers and a few homodimers. Clustering of proteins with similar interaction patterns pinpoints proteins involved in the same developmental program and provides valuable information about the participation of uncharacterized proteins in these programs. Furthermore, a model is proposed that integrates the floral induction and floral organ formation networks based on the interactions between the proteins involved. Heterodimers between flower induction and floral organ identity proteins were observed, which point to (auto)regulatory mechanisms that prevent the activity of flower induction proteins in the flower.
    Original languageEnglish
    Pages (from-to)1424-1433
    JournalThe Plant Cell
    Volume17
    Issue number5
    DOIs
    Publication statusPublished - 2005

    Keywords

    • protein-protein interactions
    • bimolecular fluorescence complementation
    • living plant-cells
    • saccharomyces-cerevisiae
    • flower development
    • homeotic gene
    • meristem identity
    • floral organ
    • ectopic expression
    • mass-spectrometry

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