Comprehensive analysis of B-Lactam antibiotics including penicillins, cephalosporins and carbapenems in poultry muscle using liquid chromatography coupled to tandem mass spectrometry

B.J.A. Berendsen, H.W. Gerritsen, R.S. Wegh, S.L. Lameris, R. van Sebille, A.A.M. Stolker, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

A comprehensive method for the quantitative residue analysis of trace levels of 22 ß-lactam antibiotics, including penicillins, cephalosporins, and carbapenems, in poultry muscle by liquid chromatography in combination with tandem mass spectrometric detection is reported. The samples analyzed for ß-lactam residues are hydrolyzed using piperidine in order to improve compound stability and to include the total residue content of the cephalosporin ceftifour. The reaction procedure was optimized using a full experimental design. Following detailed isotope labeling, tandem mass spectrometry studies and exact mass measurements using high-resolution mass spectrometry reaction schemes could be proposed for all ß-lactams studied. The main reaction occurring is the hydrolysis of the ß-lactam ring under formation of the piperidine substituted amide. For some ß-lactams, multiple isobaric hydrolysis reaction products are obtained, in accordance with expectations, but this did not hamper quantitative analysis. The final method was fully validated as a quantitative confirmatory residue analysis method according to Commission Decision 2002/657/EC and showed satisfactory quantitative performance for all compounds with trueness between 80 and 110 % and within-laboratory reproducibility below 22 % at target level, except for biapenem. For biapenem, the method proved to be suitable for qualitative analysis only.
Original languageEnglish
Pages (from-to)7859-7874
JournalAnalytical and Bioanalytical Chemistry
Volume405
Issue number24
DOIs
Publication statusPublished - 2013

Keywords

  • beta-lactamase
  • quantitative-analysis
  • ceftiofur resistance
  • escherichia-coli
  • european-union
  • bovine muscle
  • kidney tissue
  • enterobacteriaceae
  • salmonella
  • mechanisms

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