Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 7859-7874 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 405 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- beta-lactamase
- quantitative-analysis
- ceftiofur resistance
- escherichia-coli
- european-union
- bovine muscle
- kidney tissue
- enterobacteriaceae
- salmonella
- mechanisms
Cite this
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Comprehensive analysis of B-Lactam antibiotics including penicillins, cephalosporins and carbapenems in poultry muscle using liquid chromatography coupled to tandem mass spectrometry. / Berendsen, B.J.A.; Gerritsen, H.W.; Wegh, R.S.; Lameris, S.L.; van Sebille, R.; Stolker, A.A.M.; Nielen, M.W.F.
In: Analytical and Bioanalytical Chemistry, Vol. 405, No. 24, 2013, p. 7859-7874.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Comprehensive analysis of B-Lactam antibiotics including penicillins, cephalosporins and carbapenems in poultry muscle using liquid chromatography coupled to tandem mass spectrometry
AU - Berendsen, B.J.A.
AU - Gerritsen, H.W.
AU - Wegh, R.S.
AU - Lameris, S.L.
AU - van Sebille, R.
AU - Stolker, A.A.M.
AU - Nielen, M.W.F.
PY - 2013
Y1 - 2013
N2 - A comprehensive method for the quantitative residue analysis of trace levels of 22 ß-lactam antibiotics, including penicillins, cephalosporins, and carbapenems, in poultry muscle by liquid chromatography in combination with tandem mass spectrometric detection is reported. The samples analyzed for ß-lactam residues are hydrolyzed using piperidine in order to improve compound stability and to include the total residue content of the cephalosporin ceftifour. The reaction procedure was optimized using a full experimental design. Following detailed isotope labeling, tandem mass spectrometry studies and exact mass measurements using high-resolution mass spectrometry reaction schemes could be proposed for all ß-lactams studied. The main reaction occurring is the hydrolysis of the ß-lactam ring under formation of the piperidine substituted amide. For some ß-lactams, multiple isobaric hydrolysis reaction products are obtained, in accordance with expectations, but this did not hamper quantitative analysis. The final method was fully validated as a quantitative confirmatory residue analysis method according to Commission Decision 2002/657/EC and showed satisfactory quantitative performance for all compounds with trueness between 80 and 110 % and within-laboratory reproducibility below 22 % at target level, except for biapenem. For biapenem, the method proved to be suitable for qualitative analysis only.
AB - A comprehensive method for the quantitative residue analysis of trace levels of 22 ß-lactam antibiotics, including penicillins, cephalosporins, and carbapenems, in poultry muscle by liquid chromatography in combination with tandem mass spectrometric detection is reported. The samples analyzed for ß-lactam residues are hydrolyzed using piperidine in order to improve compound stability and to include the total residue content of the cephalosporin ceftifour. The reaction procedure was optimized using a full experimental design. Following detailed isotope labeling, tandem mass spectrometry studies and exact mass measurements using high-resolution mass spectrometry reaction schemes could be proposed for all ß-lactams studied. The main reaction occurring is the hydrolysis of the ß-lactam ring under formation of the piperidine substituted amide. For some ß-lactams, multiple isobaric hydrolysis reaction products are obtained, in accordance with expectations, but this did not hamper quantitative analysis. The final method was fully validated as a quantitative confirmatory residue analysis method according to Commission Decision 2002/657/EC and showed satisfactory quantitative performance for all compounds with trueness between 80 and 110 % and within-laboratory reproducibility below 22 % at target level, except for biapenem. For biapenem, the method proved to be suitable for qualitative analysis only.
KW - beta-lactamase
KW - quantitative-analysis
KW - ceftiofur resistance
KW - escherichia-coli
KW - european-union
KW - bovine muscle
KW - kidney tissue
KW - enterobacteriaceae
KW - salmonella
KW - mechanisms
U2 - 10.1007/s00216-013-6804-6
DO - 10.1007/s00216-013-6804-6
M3 - Article
VL - 405
SP - 7859
EP - 7874
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
SN - 1618-2642
IS - 24
ER -