Compositional analysis of archaeal communities in high and low microbial abundance sponges in the Misool coral reef system, Indonesia

Ana Rita Moura Polónia*, Daniel Francis Richard Cleary, Francisco José Riso da Costa Coelho, Leontine E. Becking, Nicole Joy de Voogd, Abdul Hamid A. Toha, Newton Carlos Marcial Gomes

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

The high/low microbial abundance (HMA/LMA) dichotomy in sponges has been the subject of several studies over recent years, but few studies have analysed this dichotomy in terms of the sponge archaeal community and function. Using a 16S rRNA gene barcoded pyrosequencing approach and predictive functional analysis (PICRUSt) we compared the archaeal composition, richness and predicted function of one HMA sponge (Xestospongia testudinaria), one LMA sponge (Stylissa carteri) and one sponge species of unknown microbial abundance (Aaptos lobata). Although most of the archaeal sequences were assigned to the Crenarchaeota phylum, S. carteri had the highest percentage of sequences assigned to the Euryarchaeota phylum. Variation among sponge species explained >85% of the variation in archaeal operational taxonomic unit (OTU) composition with each sponge species forming a distinct cluster. There were significant differences in predicted PICRUSt profiles among sponge species, suggesting that archaeal communities present in the studied sponge species may perform different functions. X. testudinaria and A. lobata were similar both in terms of OTU and KEGG orthologues composition, which may indicate that A. lobata is a HMA sponge. Additionally, some of the most enriched functions seem to be related to traits associated with high and low microbial abundance sponges.

Original languageEnglish
Pages (from-to)537-550
JournalMarine Biology Research
Volume14
Issue number6
DOIs
Publication statusPublished - 3 Aug 2018

Keywords

  • Aaptos lobata
  • Archaea;16S rRNA gene
  • HMA
  • LMA
  • Mathias Middelboe
  • PICRUSt

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    Polónia, A. R. M., Cleary, D. F. R., Riso da Costa Coelho, F. J., Becking, L. E., de Voogd, N. J., Toha, A. H. A., & Gomes, N. C. M. (2018). Compositional analysis of archaeal communities in high and low microbial abundance sponges in the Misool coral reef system, Indonesia. Marine Biology Research, 14(6), 537-550. https://doi.org/10.1080/17451000.2018.1498977