Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus

Ken Komatsu, Kazuo Yamashita, Kota Sugawara, Martin Verbeek, Naoko Fujita, Kaoru Hanada, Tamaki Uehara-Ichiki, Shin Ichi Fuji

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3 Citations (Scopus)

Abstract

Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus and has an exceptionally wide host range. It causes severe damage to lilies. Here we report on the complete nucleotide sequences of two new Japanese PlAMV isolates, one from the eudicot weed Viola grypoceras (PlAMV-Vi), and the other from the eudicot shrub Nandina domestica Thunb. (PlAMV-NJ). Their genomes contain five open reading frames (ORFs), which is characteristic of potexviruses. Surprisingly, the isolates showed only 76.0–78.0 % sequence identity with each other and with other PlAMV isolates, including isolates from Japanese lily and American nandina. Amino acid alignments of the replicase coding region encoded by ORF1 showed that the regions between the methyltransferase and helicase domains were less conserved than other regions, with several insertions and/or deletions. Phylogenetic analyses of the full-length nucleotide sequences revealed a moderate correlation between phylogenetic clustering and the original host plants of the PlAMV isolates. This study revealed the presence of two highly divergent PlAMV isolates in Japan.
LanguageEnglish
Pages581-584
JournalArchives of Virology
Volume162
Issue number2
DOIs
Publication statusPublished - 2017

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Plantago
Mosaic Viruses
Genome
Potexvirus
Lilium
Viola
Host Specificity
Methyltransferases
Open Reading Frames
Cluster Analysis
Japan
Amino Acids

Cite this

Komatsu, Ken ; Yamashita, Kazuo ; Sugawara, Kota ; Verbeek, Martin ; Fujita, Naoko ; Hanada, Kaoru ; Uehara-Ichiki, Tamaki ; Fuji, Shin Ichi. / Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus. In: Archives of Virology. 2017 ; Vol. 162, No. 2. pp. 581-584.
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abstract = "Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus and has an exceptionally wide host range. It causes severe damage to lilies. Here we report on the complete nucleotide sequences of two new Japanese PlAMV isolates, one from the eudicot weed Viola grypoceras (PlAMV-Vi), and the other from the eudicot shrub Nandina domestica Thunb. (PlAMV-NJ). Their genomes contain five open reading frames (ORFs), which is characteristic of potexviruses. Surprisingly, the isolates showed only 76.0–78.0 {\%} sequence identity with each other and with other PlAMV isolates, including isolates from Japanese lily and American nandina. Amino acid alignments of the replicase coding region encoded by ORF1 showed that the regions between the methyltransferase and helicase domains were less conserved than other regions, with several insertions and/or deletions. Phylogenetic analyses of the full-length nucleotide sequences revealed a moderate correlation between phylogenetic clustering and the original host plants of the PlAMV isolates. This study revealed the presence of two highly divergent PlAMV isolates in Japan.",
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Komatsu, K, Yamashita, K, Sugawara, K, Verbeek, M, Fujita, N, Hanada, K, Uehara-Ichiki, T & Fuji, SI 2017, 'Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus', Archives of Virology, vol. 162, no. 2, pp. 581-584. https://doi.org/10.1007/s00705-016-3110-6

Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus. / Komatsu, Ken; Yamashita, Kazuo; Sugawara, Kota; Verbeek, Martin; Fujita, Naoko; Hanada, Kaoru; Uehara-Ichiki, Tamaki; Fuji, Shin Ichi.

In: Archives of Virology, Vol. 162, No. 2, 2017, p. 581-584.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Komatsu, Ken

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AU - Fuji, Shin Ichi

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AB - Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus and has an exceptionally wide host range. It causes severe damage to lilies. Here we report on the complete nucleotide sequences of two new Japanese PlAMV isolates, one from the eudicot weed Viola grypoceras (PlAMV-Vi), and the other from the eudicot shrub Nandina domestica Thunb. (PlAMV-NJ). Their genomes contain five open reading frames (ORFs), which is characteristic of potexviruses. Surprisingly, the isolates showed only 76.0–78.0 % sequence identity with each other and with other PlAMV isolates, including isolates from Japanese lily and American nandina. Amino acid alignments of the replicase coding region encoded by ORF1 showed that the regions between the methyltransferase and helicase domains were less conserved than other regions, with several insertions and/or deletions. Phylogenetic analyses of the full-length nucleotide sequences revealed a moderate correlation between phylogenetic clustering and the original host plants of the PlAMV isolates. This study revealed the presence of two highly divergent PlAMV isolates in Japan.

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