Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216T

Elleke F. Bosma; Jasper J. Koehorst; Sacha A.F.T. van Hijum; Bernadet Renckens; Bastienne Vriesendorp; Tom van de Weijer; Peter J. Schaap; Willem M. de Vos; John van der Oost; Richard van Kranenburg

doi:10.1186/s40793-016-0172-8

Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216^T

Elleke F. Bosma, Jasper J. Koehorst, Sacha A.F.T. van Hijum, Bernadet Renckens, Bastienne Vriesendorp, Tom van de Weijer, Peter J. Schaap, Willem M. de Vos, John van der Oost, Richard van Kranenburg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

11 Citations (Scopus)

Abstract

Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216^T, which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.

Original language	English
Article number	52
Number of pages	11
Journal	Standards in Genomic Sciences
Volume	11
DOIs	https://doi.org/10.1186/s40793-016-0172-8
Publication status	Published - 2016

Keywords

Bacillus smithii
Biotechnology
Genome sequence
Lactic acid
Thermophile
Thermophilic bacillus

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/s40793-016-0172-8Licence: CC BY

Cite this

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title = "Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216T",

abstract = "Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216T, which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.",

keywords = "Bacillus smithii, Biotechnology, Genome sequence, Lactic acid, Thermophile, Thermophilic bacillus",

author = "Bosma, {Elleke F.} and Koehorst, {Jasper J.} and {van Hijum}, {Sacha A.F.T.} and Bernadet Renckens and Bastienne Vriesendorp and {van de Weijer}, Tom and Schaap, {Peter J.} and {de Vos}, {Willem M.} and {van der Oost}, John and {van Kranenburg}, Richard",

year = "2016",

doi = "10.1186/s40793-016-0172-8",

language = "English",

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journal = "Standards in Genomic Sciences",

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T1 - Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216T

AU - Bosma, Elleke F.

AU - Koehorst, Jasper J.

AU - van Hijum, Sacha A.F.T.

AU - Renckens, Bernadet

AU - Vriesendorp, Bastienne

AU - van de Weijer, Tom

AU - Schaap, Peter J.

AU - de Vos, Willem M.

AU - van der Oost, John

AU - van Kranenburg, Richard

PY - 2016

Y1 - 2016

N2 - Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216T, which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.

AB - Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216T, which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.

KW - Bacillus smithii

KW - Biotechnology

KW - Genome sequence

KW - Lactic acid

KW - Thermophile

KW - Thermophilic bacillus

U2 - 10.1186/s40793-016-0172-8

DO - 10.1186/s40793-016-0172-8

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