Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

K.E. Nelson; C. Weinel; I.T. Paulsen; R.J. Dodson; H. Hilbert; V.A.P. Martins dos Santos; D.E. Fouts; S.R. Gill; M. Pop; M. Holmes; L. Brinkac; M. Beanan; R.T. DeBoy; S. Daugherty; J. Kolonay; R. Madupu; W. Nelson; O. White; J. Peterson; H. Khouri; I. Hance; P. Chris Lee; E. Holtzapple; D. Scanlan; K. Tran; A. Moazzez; T. Utterback; M. Rizzo; K. Lee; D. Kosack; D. Moestl; H. Wedler; J. Lauber; D. Stjepandic; J. Hoheisel; M. Straetz; S. Heim; C. Kiewitz; J. Eisen; K.N. Timmis; A. Düsterhöft; B. Tümmler; C.M. Fraser

doi:10.1046/j.1462-2920.2002.00366.x

Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

K.E. Nelson, C. Weinel, I.T. Paulsen, R.J. Dodson, H. Hilbert, V.A.P. Martins dos Santos, D.E. Fouts, S.R. Gill, M. Pop, M. Holmes, L. Brinkac, M. Beanan, R.T. DeBoy, S. Daugherty, J. Kolonay, R. Madupu, W. Nelson, O. White, J. Peterson, H. KhouriI. Hance, P. Chris Lee, E. Holtzapple, D. Scanlan, K. Tran, A. Moazzez, T. Utterback, M. Rizzo, K. Lee, D. Kosack, D. Moestl, H. Wedler, J. Lauber, D. Stjepandic, J. Hoheisel, M. Straetz, S. Heim, C. Kiewitz, J. Eisen, K.N. Timmis, A. Düsterhöft, B. Tümmler, C.M. Fraser^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.

Original language	English
Pages (from-to)	799-808
Number of pages	10
Journal	Environmental Microbiology
Volume	4
Issue number	12
DOIs	https://doi.org/10.1046/j.1462-2920.2002.00366.x
Publication status	Published - Dec 2002
Externally published	Yes

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Nelson, K. E., Weinel, C., Paulsen, I. T., Dodson, R. J., Hilbert, H., Martins dos Santos, V. A. P., Fouts, D. E., Gill, S. R., Pop, M., Holmes, M., Brinkac, L., Beanan, M., DeBoy, R. T., Daugherty, S., Kolonay, J., Madupu, R., Nelson, W., White, O., Peterson, J., ... Fraser, C. M. (2002). Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environmental Microbiology, 4(12), 799-808. https://doi.org/10.1046/j.1462-2920.2002.00366.x

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title = "Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440",

abstract = "Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.",

author = "K.E. Nelson and C. Weinel and I.T. Paulsen and R.J. Dodson and H. Hilbert and {Martins dos Santos}, V.A.P. and D.E. Fouts and S.R. Gill and M. Pop and M. Holmes and L. Brinkac and M. Beanan and R.T. DeBoy and S. Daugherty and J. Kolonay and R. Madupu and W. Nelson and O. White and J. Peterson and H. Khouri and I. Hance and {Chris Lee}, P. and E. Holtzapple and D. Scanlan and K. Tran and A. Moazzez and T. Utterback and M. Rizzo and K. Lee and D. Kosack and D. Moestl and H. Wedler and J. Lauber and D. Stjepandic and J. Hoheisel and M. Straetz and S. Heim and C. Kiewitz and J. Eisen and K.N. Timmis and A. D{\"u}sterh{\"o}ft and B. T{\"u}mmler and C.M. Fraser",

year = "2002",

month = dec,

doi = "10.1046/j.1462-2920.2002.00366.x",

language = "English",

volume = "4",

pages = "799--808",

journal = "Environmental Microbiology",

issn = "1462-2912",

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Nelson, KE, Weinel, C, Paulsen, IT, Dodson, RJ, Hilbert, H, Martins dos Santos, VAP, Fouts, DE, Gill, SR, Pop, M, Holmes, M, Brinkac, L, Beanan, M, DeBoy, RT, Daugherty, S, Kolonay, J, Madupu, R, Nelson, W, White, O, Peterson, J, Khouri, H, Hance, I, Chris Lee, P, Holtzapple, E, Scanlan, D, Tran, K, Moazzez, A, Utterback, T, Rizzo, M, Lee, K, Kosack, D, Moestl, D, Wedler, H, Lauber, J, Stjepandic, D, Hoheisel, J, Straetz, M, Heim, S, Kiewitz, C, Eisen, J, Timmis, KN, Düsterhöft, A, Tümmler, B & Fraser, CM 2002, 'Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440', Environmental Microbiology, vol. 4, no. 12, pp. 799-808. https://doi.org/10.1046/j.1462-2920.2002.00366.x

TY - JOUR

T1 - Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

AU - Nelson, K.E.

AU - Weinel, C.

AU - Paulsen, I.T.

AU - Dodson, R.J.

AU - Hilbert, H.

AU - Martins dos Santos, V.A.P.

AU - Fouts, D.E.

AU - Gill, S.R.

AU - Pop, M.

AU - Holmes, M.

AU - Brinkac, L.

AU - Beanan, M.

AU - DeBoy, R.T.

AU - Daugherty, S.

AU - Kolonay, J.

AU - Madupu, R.

AU - Nelson, W.

AU - White, O.

AU - Peterson, J.

AU - Khouri, H.

AU - Hance, I.

AU - Chris Lee, P.

AU - Holtzapple, E.

AU - Scanlan, D.

AU - Tran, K.

AU - Moazzez, A.

AU - Utterback, T.

AU - Rizzo, M.

AU - Lee, K.

AU - Kosack, D.

AU - Moestl, D.

AU - Wedler, H.

AU - Lauber, J.

AU - Stjepandic, D.

AU - Hoheisel, J.

AU - Straetz, M.

AU - Heim, S.

AU - Kiewitz, C.

AU - Eisen, J.

AU - Timmis, K.N.

AU - Düsterhöft, A.

AU - Tümmler, B.

AU - Fraser, C.M.

PY - 2002/12

Y1 - 2002/12

N2 - Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.

AB - Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non-pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.

U2 - 10.1046/j.1462-2920.2002.00366.x

DO - 10.1046/j.1462-2920.2002.00366.x

M3 - Article

C2 - 12534463

AN - SCOPUS:0036933705

SN - 1462-2912

VL - 4

SP - 799

EP - 808

JO - Environmental Microbiology

JF - Environmental Microbiology

IS - 12

ER -

Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

Abstract

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