Complete biological reductive transformation of tetrachloroethene to ethane.

W.P. de Bruin, M.J.J. Kotterman, M.A. Posthumus, G. Schraa, A.J.B. Zehnder

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256 Citations (Scopus)

Abstract

Reductive dechlorination of tetrachloroethene (perchloroethylene; PCE) was observed at 20 degrees C in a fixed-bed column, filled with a mixture (3:1) of anaerobic sediment from the Rhine river and anaerobic granular sludge. In the presence of lactate (1 mM) as an electron donor, 9 microM PCE was dechlorinated to ethene. Ethene was further reduced to ethane. Mass balances demonstrated an almost complete conversion (95 to 98%), with no chlorinated compounds remaining (less than 0.5 micrograms/liter). When the temperature was lowered to 10 degrees C, an adaptation of 2 weeks was necessary to obtain the same performance as at 20 degrees C. Dechlorination by column material to ethene, followed by a slow ethane production, could also be achieved in batch cultures. Ethane was not formed in the presence of bromoethanesulfonic acid, an inhibitor of methanogenesis. The high dechlorination rate (3.7 mumol.l-1.h-1), even at low temperatures and considerable PCE concentrations, together with the absence of chlorinated end products, makes reductive dechlorination an attractive method for removal of PCE in bioremediation processes.
Original languageEnglish
Pages (from-to)1996-2000
JournalApplied and Environmental Microbiology
Volume58
Issue number6
Publication statusPublished - 1992

Keywords

  • tetrachloroethylene
  • ethane
  • river rhine
  • water bottoms

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