Comparison of three non-invasive methods, including challenge tests, to measure metabolic health in mice using indirect calorimetry

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Indirect calorimetry (InCa) is a non-invasive method that can potentially be used to assess metabolic health. To test this, we compared three InCa-based methods using aged and adult C57BL/6J male mice (72 and 10 weeks old) and assessed the stability of the responses over time. The aged and adult mice differed in adiposity and white adipose tissue mitochondrial density, indicative of a compromised metabolic health of the aged mice. In method 1, diurnal patterns of respiratory exchange ratio (RER) were followed for 24hrs under standard conditions. In method 2, fasted mice received glucose to test effective switching in substrate oxidation. In method 3, mice were exposed to oxygen restriction (OxR, 14.5 % O2) to test how effectively animals switch from normal to low oxygen availability. Each method was repeated with at least a 10 week interval to assess response stability. Method 1 was not stable in time. Method 2 did not reveal differences in metabolic health between adult and aged mice. Method 3 appeared most sensitive to detect differences between both groups and in each period. Adult mice maintained reduced oxygen consumption under OxR, while aged mice did not. Thus, the response to OxR is a sensitive and reproducible method to non-invasively measure metabolic health in mice. Molecular analyses showed that OxR affected glucose and lactate metabolic pathways in liver and adipose tissue, supporting the observed differences in oxygen consumption.
Original languageEnglish
Title of host publicationAbstracts 20th International Congress of Nutrition
EditorsB. Koletzko
Place of PublicationBasel
PublisherKarger
Pages8-8
Volume63
ISBN (Print)9783318025163
Publication statusPublished - 2013
Event20th International Congress of Nutrition, Granada, Spain -
Duration: 15 Sep 201320 Sep 2013

Conference

Conference20th International Congress of Nutrition, Granada, Spain
Period15/09/1320/09/13

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