Comparison of the functional properties of RuBisCO protein isolate extracted from sugar beet leaves with commercial whey protein and soy protein isolates

Anneke H. Martin, Oscar Castellani*, Govardus A.H. de Jong, Lionel Bovetto, Christophe Schmitt

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Background: RuBisCO was extracted from sugar beet leaves using soft and food-compatible technologies. Proximate composition, solubility, emulsifying, foaming and gelling properties of the protein isolate were determined. All these properties were systematically benchmarked against commercial whey and soy protein isolates used in food applications. Results: RuBisCO protein isolate (RPI) contained 930 g kg−1 of crude protein. Protein solubility was higher than 80% at pH values lower than 4.0 or higher than 5.5. Foaming capacity of RPI was better at pH 4.0 than at pH 7.0. Interestingly, 10 g kg−1 protein foams were more stable (pH 7.0 and 4.0) than foams obtained with whey or soy protein. Moreover, 10 g kg−1 RPI emulsions at pH 4.0 or 7.0 exhibited good stability, being similar to whey protein isolate. Remarkable gelling properties were observed at pH 7.0, where 50 g kg−1 protein solutions of RPI formed self-supporting gels while more concentrated solutions were needed for whey or soy protein. Conclusion: RuBisCO showed comparable or superior functional properties to those of currently used whey and soy protein isolates. These results highlight the high potential of sugar beet leaf protein isolate as a nutritious and functional food ingredient to face global food security and protein supply.

Original languageEnglish
Pages (from-to)1568-1576
JournalJournal of the Science of Food and Agriculture
Volume99
Issue number4
Early online date25 Aug 2018
DOIs
Publication statusPublished - Mar 2019

Fingerprint

Soybean Proteins
Beta vulgaris
soy protein isolate
protein isolates
whey protein
sugar beet
functional properties
whey protein isolate
gelling properties
leaves
Proteins
foams
soy protein
foaming capacity
foaming properties
leaf protein
emulsifying properties
proteins
protein solubility
Solubility

Keywords

  • emulsion
  • gel
  • green leaf protein
  • solubility
  • sugar beet

Cite this

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title = "Comparison of the functional properties of RuBisCO protein isolate extracted from sugar beet leaves with commercial whey protein and soy protein isolates",
abstract = "Background: RuBisCO was extracted from sugar beet leaves using soft and food-compatible technologies. Proximate composition, solubility, emulsifying, foaming and gelling properties of the protein isolate were determined. All these properties were systematically benchmarked against commercial whey and soy protein isolates used in food applications. Results: RuBisCO protein isolate (RPI) contained 930 g kg−1 of crude protein. Protein solubility was higher than 80{\%} at pH values lower than 4.0 or higher than 5.5. Foaming capacity of RPI was better at pH 4.0 than at pH 7.0. Interestingly, 10 g kg−1 protein foams were more stable (pH 7.0 and 4.0) than foams obtained with whey or soy protein. Moreover, 10 g kg−1 RPI emulsions at pH 4.0 or 7.0 exhibited good stability, being similar to whey protein isolate. Remarkable gelling properties were observed at pH 7.0, where 50 g kg−1 protein solutions of RPI formed self-supporting gels while more concentrated solutions were needed for whey or soy protein. Conclusion: RuBisCO showed comparable or superior functional properties to those of currently used whey and soy protein isolates. These results highlight the high potential of sugar beet leaf protein isolate as a nutritious and functional food ingredient to face global food security and protein supply.",
keywords = "emulsion, gel, green leaf protein, solubility, sugar beet",
author = "Martin, {Anneke H.} and Oscar Castellani and {de Jong}, {Govardus A.H.} and Lionel Bovetto and Christophe Schmitt",
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Comparison of the functional properties of RuBisCO protein isolate extracted from sugar beet leaves with commercial whey protein and soy protein isolates. / Martin, Anneke H.; Castellani, Oscar; de Jong, Govardus A.H.; Bovetto, Lionel; Schmitt, Christophe.

In: Journal of the Science of Food and Agriculture, Vol. 99, No. 4, 03.2019, p. 1568-1576.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Comparison of the functional properties of RuBisCO protein isolate extracted from sugar beet leaves with commercial whey protein and soy protein isolates

AU - Martin, Anneke H.

AU - Castellani, Oscar

AU - de Jong, Govardus A.H.

AU - Bovetto, Lionel

AU - Schmitt, Christophe

PY - 2019/3

Y1 - 2019/3

N2 - Background: RuBisCO was extracted from sugar beet leaves using soft and food-compatible technologies. Proximate composition, solubility, emulsifying, foaming and gelling properties of the protein isolate were determined. All these properties were systematically benchmarked against commercial whey and soy protein isolates used in food applications. Results: RuBisCO protein isolate (RPI) contained 930 g kg−1 of crude protein. Protein solubility was higher than 80% at pH values lower than 4.0 or higher than 5.5. Foaming capacity of RPI was better at pH 4.0 than at pH 7.0. Interestingly, 10 g kg−1 protein foams were more stable (pH 7.0 and 4.0) than foams obtained with whey or soy protein. Moreover, 10 g kg−1 RPI emulsions at pH 4.0 or 7.0 exhibited good stability, being similar to whey protein isolate. Remarkable gelling properties were observed at pH 7.0, where 50 g kg−1 protein solutions of RPI formed self-supporting gels while more concentrated solutions were needed for whey or soy protein. Conclusion: RuBisCO showed comparable or superior functional properties to those of currently used whey and soy protein isolates. These results highlight the high potential of sugar beet leaf protein isolate as a nutritious and functional food ingredient to face global food security and protein supply.

AB - Background: RuBisCO was extracted from sugar beet leaves using soft and food-compatible technologies. Proximate composition, solubility, emulsifying, foaming and gelling properties of the protein isolate were determined. All these properties were systematically benchmarked against commercial whey and soy protein isolates used in food applications. Results: RuBisCO protein isolate (RPI) contained 930 g kg−1 of crude protein. Protein solubility was higher than 80% at pH values lower than 4.0 or higher than 5.5. Foaming capacity of RPI was better at pH 4.0 than at pH 7.0. Interestingly, 10 g kg−1 protein foams were more stable (pH 7.0 and 4.0) than foams obtained with whey or soy protein. Moreover, 10 g kg−1 RPI emulsions at pH 4.0 or 7.0 exhibited good stability, being similar to whey protein isolate. Remarkable gelling properties were observed at pH 7.0, where 50 g kg−1 protein solutions of RPI formed self-supporting gels while more concentrated solutions were needed for whey or soy protein. Conclusion: RuBisCO showed comparable or superior functional properties to those of currently used whey and soy protein isolates. These results highlight the high potential of sugar beet leaf protein isolate as a nutritious and functional food ingredient to face global food security and protein supply.

KW - emulsion

KW - gel

KW - green leaf protein

KW - solubility

KW - sugar beet

U2 - 10.1002/jsfa.9335

DO - 10.1002/jsfa.9335

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SN - 0022-5142

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