Comparison of Protein Extracts from Various Unicellular Green Sources

Emma Teuling, Peter A. Wierenga, Johan W. Schrama, Harry Gruppen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources.
Original languageEnglish
Pages (from-to)7989-8002
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number36
DOIs
Publication statusPublished - 2017

Fingerprint

protein isolates
extracts
protein sources
Proteins
proteins
Spirulina maxima
Arthrospira
protein content
Tetraselmis
Nannochloropsis
Scenedesmus
protein solubility
isolation techniques
autotrophs
ionic strength
Spirulina
Ionic strength
Osmolar Concentration
Solubility
Chemical analysis

Keywords

  • amino acid composition
  • carbohydrate composition
  • cyanobacteria
  • Microalgae
  • physicochemical properties
  • single-cell protein

Cite this

@article{a5d2b0639633401daf69d313f5f98a79,
title = "Comparison of Protein Extracts from Various Unicellular Green Sources",
abstract = "Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62{\%} w/w) and in protein extractability (17-74{\%} w/w), final protein isolates were obtained that had similar protein contents (62-77{\%} w/w) and protein yields (3-9{\%} w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources.",
keywords = "amino acid composition, carbohydrate composition, cyanobacteria, Microalgae, physicochemical properties, single-cell protein",
author = "Emma Teuling and Wierenga, {Peter A.} and Schrama, {Johan W.} and Harry Gruppen",
year = "2017",
doi = "10.1021/acs.jafc.7b01788",
language = "English",
volume = "65",
pages = "7989--8002",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "36",

}

Comparison of Protein Extracts from Various Unicellular Green Sources. / Teuling, Emma; Wierenga, Peter A.; Schrama, Johan W.; Gruppen, Harry.

In: Journal of Agricultural and Food Chemistry, Vol. 65, No. 36, 2017, p. 7989-8002.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Comparison of Protein Extracts from Various Unicellular Green Sources

AU - Teuling, Emma

AU - Wierenga, Peter A.

AU - Schrama, Johan W.

AU - Gruppen, Harry

PY - 2017

Y1 - 2017

N2 - Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources.

AB - Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources.

KW - amino acid composition

KW - carbohydrate composition

KW - cyanobacteria

KW - Microalgae

KW - physicochemical properties

KW - single-cell protein

U2 - 10.1021/acs.jafc.7b01788

DO - 10.1021/acs.jafc.7b01788

M3 - Article

VL - 65

SP - 7989

EP - 8002

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 36

ER -