Comparison of nuclear replication activity and protein expression patterns during tomato seed germination

R.J. Bino, J.H.W. Bergervoet, C.H.R. de Vos, H.L. Kraak, S. Lanteri, W.J. van der Burg, X.Y. Zheng

    Research output: Contribution to journalArticleAcademicpeer-review

    11 Citations (Scopus)

    Abstract

    Flow cytometric determination of DNA amounts in embryos of fully matured dry tomato (Lycopersicon esculentum Mill.) seeds revealed mainly 2C signals. Apparently, most embryonic cells had arrested the cell cycle activity during maturation at the presynthetic G1 phase of nuclear division. During imbibition of tomato seeds, both in water and in PEG solution, an augmentation in the 4C signal of tissues at the embryonic root tip region occurred. This increase was found to be related to cells entering the synthetic phase of nuclear division leading to a doubling of the chromosomal material. In the root tip cells, 4C/2C ratios rose 1 day after imbibition in water while radicle emergence started 2 days later. Apparently, DNA synthesis preceded germination. The induction of nuclear activities was compared with changes in protein expression patterns during seed imbibition. Using chemiluminescent immunodetection after Western blotting, we identified changes in /3-tubulin expression during germination and priming of tomato seeds. Tubulin was found to be synthesized 8 h after the start of imbibition both in water and in PEG. The start of tubulin synthesis considerably preceded DNA replication, cell division and germination. The induction of cell cycle activities may be an early indicator of the initiation of cell division and the start of germination.
    LanguageEnglish
    Pages71-77
    JournalField Crops Research
    Volume45
    Issue number1-3
    DOIs
    Publication statusPublished - 1996

    Fingerprint

    imbibition
    germination
    seed germination
    protein synthesis
    tubulin
    tomatoes
    seed
    protein
    seeds
    DNA
    root tips
    cell division
    cell cycle
    root radicle
    synthesis
    water
    DNA replication
    embryo (plant)
    interphase
    Solanum lycopersicum var. lycopersicum

    Cite this

    @article{8708996283a14cefab1c34ca20122cd5,
    title = "Comparison of nuclear replication activity and protein expression patterns during tomato seed germination",
    abstract = "Flow cytometric determination of DNA amounts in embryos of fully matured dry tomato (Lycopersicon esculentum Mill.) seeds revealed mainly 2C signals. Apparently, most embryonic cells had arrested the cell cycle activity during maturation at the presynthetic G1 phase of nuclear division. During imbibition of tomato seeds, both in water and in PEG solution, an augmentation in the 4C signal of tissues at the embryonic root tip region occurred. This increase was found to be related to cells entering the synthetic phase of nuclear division leading to a doubling of the chromosomal material. In the root tip cells, 4C/2C ratios rose 1 day after imbibition in water while radicle emergence started 2 days later. Apparently, DNA synthesis preceded germination. The induction of nuclear activities was compared with changes in protein expression patterns during seed imbibition. Using chemiluminescent immunodetection after Western blotting, we identified changes in /3-tubulin expression during germination and priming of tomato seeds. Tubulin was found to be synthesized 8 h after the start of imbibition both in water and in PEG. The start of tubulin synthesis considerably preceded DNA replication, cell division and germination. The induction of cell cycle activities may be an early indicator of the initiation of cell division and the start of germination.",
    author = "R.J. Bino and J.H.W. Bergervoet and {de Vos}, C.H.R. and H.L. Kraak and S. Lanteri and {van der Burg}, W.J. and X.Y. Zheng",
    year = "1996",
    doi = "10.1016/0378-4290(95)00060-7",
    language = "English",
    volume = "45",
    pages = "71--77",
    journal = "Field Crops Research",
    issn = "0378-4290",
    publisher = "Elsevier",
    number = "1-3",

    }

    Comparison of nuclear replication activity and protein expression patterns during tomato seed germination. / Bino, R.J.; Bergervoet, J.H.W.; de Vos, C.H.R.; Kraak, H.L.; Lanteri, S.; van der Burg, W.J.; Zheng, X.Y.

    In: Field Crops Research, Vol. 45, No. 1-3, 1996, p. 71-77.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Comparison of nuclear replication activity and protein expression patterns during tomato seed germination

    AU - Bino, R.J.

    AU - Bergervoet, J.H.W.

    AU - de Vos, C.H.R.

    AU - Kraak, H.L.

    AU - Lanteri, S.

    AU - van der Burg, W.J.

    AU - Zheng, X.Y.

    PY - 1996

    Y1 - 1996

    N2 - Flow cytometric determination of DNA amounts in embryos of fully matured dry tomato (Lycopersicon esculentum Mill.) seeds revealed mainly 2C signals. Apparently, most embryonic cells had arrested the cell cycle activity during maturation at the presynthetic G1 phase of nuclear division. During imbibition of tomato seeds, both in water and in PEG solution, an augmentation in the 4C signal of tissues at the embryonic root tip region occurred. This increase was found to be related to cells entering the synthetic phase of nuclear division leading to a doubling of the chromosomal material. In the root tip cells, 4C/2C ratios rose 1 day after imbibition in water while radicle emergence started 2 days later. Apparently, DNA synthesis preceded germination. The induction of nuclear activities was compared with changes in protein expression patterns during seed imbibition. Using chemiluminescent immunodetection after Western blotting, we identified changes in /3-tubulin expression during germination and priming of tomato seeds. Tubulin was found to be synthesized 8 h after the start of imbibition both in water and in PEG. The start of tubulin synthesis considerably preceded DNA replication, cell division and germination. The induction of cell cycle activities may be an early indicator of the initiation of cell division and the start of germination.

    AB - Flow cytometric determination of DNA amounts in embryos of fully matured dry tomato (Lycopersicon esculentum Mill.) seeds revealed mainly 2C signals. Apparently, most embryonic cells had arrested the cell cycle activity during maturation at the presynthetic G1 phase of nuclear division. During imbibition of tomato seeds, both in water and in PEG solution, an augmentation in the 4C signal of tissues at the embryonic root tip region occurred. This increase was found to be related to cells entering the synthetic phase of nuclear division leading to a doubling of the chromosomal material. In the root tip cells, 4C/2C ratios rose 1 day after imbibition in water while radicle emergence started 2 days later. Apparently, DNA synthesis preceded germination. The induction of nuclear activities was compared with changes in protein expression patterns during seed imbibition. Using chemiluminescent immunodetection after Western blotting, we identified changes in /3-tubulin expression during germination and priming of tomato seeds. Tubulin was found to be synthesized 8 h after the start of imbibition both in water and in PEG. The start of tubulin synthesis considerably preceded DNA replication, cell division and germination. The induction of cell cycle activities may be an early indicator of the initiation of cell division and the start of germination.

    U2 - 10.1016/0378-4290(95)00060-7

    DO - 10.1016/0378-4290(95)00060-7

    M3 - Article

    VL - 45

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    EP - 77

    JO - Field Crops Research

    T2 - Field Crops Research

    JF - Field Crops Research

    SN - 0378-4290

    IS - 1-3

    ER -