Comparison of levels of genetic diversity detected with AFLP and microsatellite markers within and among mixed Q. petraea (MATT.) LIEBL. and Q. robur L. stands

S. Mariette, J. Cottrell, U.M. Csaikl, P. Goikoechea, A. Konig, A.J. Lowe, B.C. van Dam, T. Barreneche, C. Bodenes, R. Streiff, K. Burg, K. Groppe, R.C. Munro, H. Tabbener, A. Kremer

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    Abstract

    The aim of this study was to compare genetic diversity within and among Quercus spp. populations based on two contrasting types of nuclear markers. Seven mixed stands of Quercus petraea and Quercus robur were analysed using six highly polymorphic and codominantly inherited microsatellite markers as well as 155 dominant AFLP markers. Genetic differentiation and genetic diversity within each population were assessed. The intra- and inter-locus variances were calculated, and the results were used to compare the genetic diversity between populations. Both classes of markers revealed similar results: the genetic diversity within population and the genetic differentiation among populations is greater in Q. petraea than in Q. robur The genetic differentiation is generally higher when AFLP markers were used in comparison to microsatellites. For AFLPs, the inter-locus variance is always much higher than the intra-locus variance, and explains why it was not possible to distinguish populations using this marker system. Finally, no significant positive correlation was found between the level of within-population diversity assessed with the two markers.
    Original languageEnglish
    Pages (from-to)72-79
    JournalSilvae genetica
    Volume51
    Issue number2/3
    Publication statusPublished - 2002

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    Microsatellite Repeats
    amplified fragment length polymorphism
    microsatellite repeats
    Quercus
    genetic variation
    Population
    genetic differentiation
    loci
    Quercus petraea
    Quercus robur
    Population Genetics
    mixed stands
    comparison
    genetic diversity
    marker

    Keywords

      Cite this

      Mariette, S., Cottrell, J., Csaikl, U. M., Goikoechea, P., Konig, A., Lowe, A. J., ... Kremer, A. (2002). Comparison of levels of genetic diversity detected with AFLP and microsatellite markers within and among mixed Q. petraea (MATT.) LIEBL. and Q. robur L. stands. Silvae genetica, 51(2/3), 72-79.
      Mariette, S. ; Cottrell, J. ; Csaikl, U.M. ; Goikoechea, P. ; Konig, A. ; Lowe, A.J. ; van Dam, B.C. ; Barreneche, T. ; Bodenes, C. ; Streiff, R. ; Burg, K. ; Groppe, K. ; Munro, R.C. ; Tabbener, H. ; Kremer, A. / Comparison of levels of genetic diversity detected with AFLP and microsatellite markers within and among mixed Q. petraea (MATT.) LIEBL. and Q. robur L. stands. In: Silvae genetica. 2002 ; Vol. 51, No. 2/3. pp. 72-79.
      @article{a451c10523c64be89d379a98fb83c257,
      title = "Comparison of levels of genetic diversity detected with AFLP and microsatellite markers within and among mixed Q. petraea (MATT.) LIEBL. and Q. robur L. stands",
      abstract = "The aim of this study was to compare genetic diversity within and among Quercus spp. populations based on two contrasting types of nuclear markers. Seven mixed stands of Quercus petraea and Quercus robur were analysed using six highly polymorphic and codominantly inherited microsatellite markers as well as 155 dominant AFLP markers. Genetic differentiation and genetic diversity within each population were assessed. The intra- and inter-locus variances were calculated, and the results were used to compare the genetic diversity between populations. Both classes of markers revealed similar results: the genetic diversity within population and the genetic differentiation among populations is greater in Q. petraea than in Q. robur The genetic differentiation is generally higher when AFLP markers were used in comparison to microsatellites. For AFLPs, the inter-locus variance is always much higher than the intra-locus variance, and explains why it was not possible to distinguish populations using this marker system. Finally, no significant positive correlation was found between the level of within-population diversity assessed with the two markers.",
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      author = "S. Mariette and J. Cottrell and U.M. Csaikl and P. Goikoechea and A. Konig and A.J. Lowe and {van Dam}, B.C. and T. Barreneche and C. Bodenes and R. Streiff and K. Burg and K. Groppe and R.C. Munro and H. Tabbener and A. Kremer",
      year = "2002",
      language = "English",
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      Mariette, S, Cottrell, J, Csaikl, UM, Goikoechea, P, Konig, A, Lowe, AJ, van Dam, BC, Barreneche, T, Bodenes, C, Streiff, R, Burg, K, Groppe, K, Munro, RC, Tabbener, H & Kremer, A 2002, 'Comparison of levels of genetic diversity detected with AFLP and microsatellite markers within and among mixed Q. petraea (MATT.) LIEBL. and Q. robur L. stands' Silvae genetica, vol. 51, no. 2/3, pp. 72-79.

      Comparison of levels of genetic diversity detected with AFLP and microsatellite markers within and among mixed Q. petraea (MATT.) LIEBL. and Q. robur L. stands. / Mariette, S.; Cottrell, J.; Csaikl, U.M.; Goikoechea, P.; Konig, A.; Lowe, A.J.; van Dam, B.C.; Barreneche, T.; Bodenes, C.; Streiff, R.; Burg, K.; Groppe, K.; Munro, R.C.; Tabbener, H.; Kremer, A.

      In: Silvae genetica, Vol. 51, No. 2/3, 2002, p. 72-79.

      Research output: Contribution to journalArticleAcademicpeer-review

      TY - JOUR

      T1 - Comparison of levels of genetic diversity detected with AFLP and microsatellite markers within and among mixed Q. petraea (MATT.) LIEBL. and Q. robur L. stands

      AU - Mariette, S.

      AU - Cottrell, J.

      AU - Csaikl, U.M.

      AU - Goikoechea, P.

      AU - Konig, A.

      AU - Lowe, A.J.

      AU - van Dam, B.C.

      AU - Barreneche, T.

      AU - Bodenes, C.

      AU - Streiff, R.

      AU - Burg, K.

      AU - Groppe, K.

      AU - Munro, R.C.

      AU - Tabbener, H.

      AU - Kremer, A.

      PY - 2002

      Y1 - 2002

      N2 - The aim of this study was to compare genetic diversity within and among Quercus spp. populations based on two contrasting types of nuclear markers. Seven mixed stands of Quercus petraea and Quercus robur were analysed using six highly polymorphic and codominantly inherited microsatellite markers as well as 155 dominant AFLP markers. Genetic differentiation and genetic diversity within each population were assessed. The intra- and inter-locus variances were calculated, and the results were used to compare the genetic diversity between populations. Both classes of markers revealed similar results: the genetic diversity within population and the genetic differentiation among populations is greater in Q. petraea than in Q. robur The genetic differentiation is generally higher when AFLP markers were used in comparison to microsatellites. For AFLPs, the inter-locus variance is always much higher than the intra-locus variance, and explains why it was not possible to distinguish populations using this marker system. Finally, no significant positive correlation was found between the level of within-population diversity assessed with the two markers.

      AB - The aim of this study was to compare genetic diversity within and among Quercus spp. populations based on two contrasting types of nuclear markers. Seven mixed stands of Quercus petraea and Quercus robur were analysed using six highly polymorphic and codominantly inherited microsatellite markers as well as 155 dominant AFLP markers. Genetic differentiation and genetic diversity within each population were assessed. The intra- and inter-locus variances were calculated, and the results were used to compare the genetic diversity between populations. Both classes of markers revealed similar results: the genetic diversity within population and the genetic differentiation among populations is greater in Q. petraea than in Q. robur The genetic differentiation is generally higher when AFLP markers were used in comparison to microsatellites. For AFLPs, the inter-locus variance is always much higher than the intra-locus variance, and explains why it was not possible to distinguish populations using this marker system. Finally, no significant positive correlation was found between the level of within-population diversity assessed with the two markers.

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      KW - genetica

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      VL - 51

      SP - 72

      EP - 79

      JO - Silvae genetica

      JF - Silvae genetica

      SN - 0037-5349

      IS - 2/3

      ER -