Comparison of Caco-2, IEC-18 and HCEC cell lines as a model for intestinal absorption of genistein, daidzein and their glycodides

A. Steensma, H.P.J.M. Noteborn, H.A. Kuiper

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Genistein and daidzein receive much attention because of their potential to prevent hormone-related cancer and cardiovascular diseases. Limited information is available on the pharmacokinetics of these compounds like, for instance, intestinal uptake by humans and systematic bioavailability. In this study the transport and metabolism of genistein, daidzein and their glycosides has been compared in various cellular models for intestinal absorption such as human colonic Caco-2, rat small intestinal IEC-18 and human immortalized colon HCEC cell lines. Genistein and daidzein were taken up by Caco-2, IEC-18 and HCEC cells and transported to almost same rate and extents. Glycosides were transported across IEC-18 and HCEC monolayers, but not across Caco-2 cells. In Caco-2 and IEC-18 cells, the glycosides were metabolized to their respective aglycones. Furthermore, it was shown that genistein and daidzein were glucuronidated and sulfated in Caco-2 cells, to glucuronidated forms in IEC-18 cells and to sulfated conjugates in HCEC cells. The results of this study compared with reported in vivo data indicate that Caco-2 cells are a valuable model for studying intestinal transport and metabolism of isoflavones.
Original languageEnglish
Pages (from-to)131-139
JournalEnvironmental Toxicology and Pharmacology
Volume16
Issue number3
DOIs
Publication statusPublished - 2004

Keywords

  • flavonoid quercetin 4'-beta-glucoside
  • in-vitro
  • phyto-estrogens
  • tight junctions
  • drug transport
  • permeability
  • rat
  • glucosides
  • monolayers
  • culture

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