Comparison of analytical and semi-preparative columns for high-performance liquid chromatography-solid-phase extraction-nuclear magnetic resonance

G. Miliauskas, T.A. van Beek, P. de Waard, R.P. Venskutonis, E.J.R. Sudhölter

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

The application of analytical and semi-preparative columns in reversed-phase liquid chromatography-solid-phase extraction-nuclear magnetic resonance (HPLC-SPE-NMR) was compared. The work was aiming at separating a higher sample amount in a single run and in this way to reduce the necessary NMR measurement time of separated compounds. Several parameters for compound separation and trapping procedures were optimised: flow rate of HPLC and make-up water pumps, choice of stationary phase cartridges and drying time. The separation and loadability of nine model compounds on analytical and semi-preparative columns was determined, as well as the focussing capacity of SH-type SPE cartridges. It was found that a semi-preparative column-or multiple peak trapping on analytical columns-gave better results than a standard 4.6mm analytical column for non-polar compounds (e.g. flavonoid aglycones, sesquiterpene lactones, non-polar terpenes, logP>2), but for polar compounds (logP
Original languageEnglish
Pages (from-to)276-284
JournalJournal of Chromatography. A, Including electrophoresis and other separation methods
Volume1112
Issue number1-2
DOIs
Publication statusPublished - 2006

Keywords

  • natural-products research
  • hplc-spe-nmr
  • plant-extracts
  • identification
  • hyphenation
  • ms
  • spectroscopy
  • probe
  • technology
  • system

Fingerprint Dive into the research topics of 'Comparison of analytical and semi-preparative columns for high-performance liquid chromatography-solid-phase extraction-nuclear magnetic resonance'. Together they form a unique fingerprint.

Cite this