Comparison of a fluoroquinolone surface plasmon resonance biosensor screening assay with established methods

S. Weigel, M.G. Pikkemaat, J.W.A. Elferink, P.P.J. Mulder, A.C. Huet, P. Delahaut, S. Schittko, R. Flerus, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

The performance of a previously developed immunochemical biosensor screening method for fluoroquinolone (FQ) antibiotics in poultry muscle, fish and egg was compared with established methods. Blank sample material of the target matrices was individually spiked with the FQs at half maximum residue levels. Homogeneity of the test materials was confirmed by liquid chromatography-mass spectrometry (LC-MS/MS). Identical sets of spiked samples as well as incurred samples from a previous feeding experiment were sent to three independent laboratories and analysed by LC-MS/MS, a microbiological assay and the new biosensor assay. The new method correctly identified all contaminated samples and demonstrated advantages in sensitivity and analysis time compared to the microbiological screening assay
Original languageEnglish
Pages (from-to)441-452
JournalFood Additives and Contaminants
Volume26
Issue number4
DOIs
Publication statusPublished - 2009

Keywords

  • veterinary drug residues
  • beta-lactam antibiotics
  • quinolone residues
  • inhibition tests
  • poultry meat
  • muscle
  • eggs
  • ciprofloxacin
  • enrofloxacin
  • products

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