Comparison between stearoyl-CoA desaturase expression in milk somatic cells and in mammary tissue of lactating dairy cows

A.A.A. Jacobs, J. Dijkstra, W.H. Hendriks, J. van Baal, A.M. van Vuuren

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Stearoyl-CoA desaturase (SCD) is an important enzyme in the bovine mammary gland, where it inserts a cis-double bond at the ¿9 position in a wide range of fatty acids. Investigating SCD expression in the bovine mammary gland generally requires invasive biopsy to obtain mammary tissue. The aim of this study was to evaluate the use of milk somatic cells as a non-invasive alternative to biopsy for measuring mammary SCD expression in dairy cows. Both milk somatic cells and mammary tissue were collected from 14 Holstein-Friesian cows and used for analysis of SCD expression by real-time PCR. The SCD5 mRNA levels in mammary tissue compared with SCD1 were low, and for several milk somatic cell samples, SCD5 expression was even below the limit of detection. A significant relationship was found between SCD1 expression in milk somatic cells and in mammary tissue. In addition, SCD1 expression in milk somatic cells was significantly related to ¿9-desaturase indices in milk, which are commonly used as an indicator of SCD1 activity within the mammary gland. Our study showed that milk somatic cells can be used as a source of mRNA to study SCD1 expression in dairy cows, offering a non-invasive alternative to mammary tissue samples obtained by biopsy.
Original languageEnglish
Pages (from-to)353-362
JournalJournal of Animal Physiology and Animal Nutrition
Volume97
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • conjugated linoleic-acid
  • epithelial-cells
  • messenger-rna
  • short communication
  • lipid-metabolism
  • gene-expression
  • linseed oil
  • gland
  • fat
  • cla

Fingerprint Dive into the research topics of 'Comparison between stearoyl-CoA desaturase expression in milk somatic cells and in mammary tissue of lactating dairy cows'. Together they form a unique fingerprint.

Cite this