Comparing artificial and natural selection in rate of adaptation to genetic stress in Aspergillus nidulans

S.E. Schoustra, S.M. Slakhorst-Wandel, A.J.M. Debets, R.F. Hoekstra

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

In an experimental study of adaptation to negative pleiotropic effects of a major fungicide resistance mutation in the filamentous fungus Aspergillus nidulans we have investigated the relative effectiveness of artificial selection vs. natural selection on the rate of compensatory evolution. Using mycelial growth rate as a fitness measure, artificial selection involved the weekly transfer of the fastest growing sector onto a fresh plate. Natural selection was approximated by transferring random samples of all the spores produced by the mycelium. Fungicide resistant and fungicide sensitive haploid and diploid strains were used in an evolution experiment over 10 weekly transfers, which is equivalent to 1200 cell cycles. Two different environmental conditions were applied: a constant fungicide-free environment and a weekly alternation between presence and absence of fungicide. Results show that for all strains and conditions used the transfer of a random sample of all spores leads to more rapid adaptation than the transfer of the visually ‘fittest’ sector. The rates of compensatory evolution in the constant and the alternating environment did not differ. Moreover, haploid strains tend to have a higher rate of adaptation than isogenic diploid strains.
Original languageEnglish
Pages (from-to)771-778
JournalJournal of Evolutionary Biology
Volume18
Issue number4
DOIs
Publication statusPublished - 2005

Fingerprint

Aspergillus nidulans
artificial selection
fungicide
natural selection
fungicides
haploidy
diploidy
spores
spore
fungicide resistance
mycelium
cell cycle
mutation
sampling
fitness
experimental study
environmental factors
fungi
environmental conditions
rate

Keywords

  • deleterious mutations
  • filamentous fungi
  • escherichia-coli
  • evolution
  • pyrimethanil
  • populations
  • fludioxonil
  • bacteria

Cite this

@article{edba0f88bc2044bdbbd1d278e015d924,
title = "Comparing artificial and natural selection in rate of adaptation to genetic stress in Aspergillus nidulans",
abstract = "In an experimental study of adaptation to negative pleiotropic effects of a major fungicide resistance mutation in the filamentous fungus Aspergillus nidulans we have investigated the relative effectiveness of artificial selection vs. natural selection on the rate of compensatory evolution. Using mycelial growth rate as a fitness measure, artificial selection involved the weekly transfer of the fastest growing sector onto a fresh plate. Natural selection was approximated by transferring random samples of all the spores produced by the mycelium. Fungicide resistant and fungicide sensitive haploid and diploid strains were used in an evolution experiment over 10 weekly transfers, which is equivalent to 1200 cell cycles. Two different environmental conditions were applied: a constant fungicide-free environment and a weekly alternation between presence and absence of fungicide. Results show that for all strains and conditions used the transfer of a random sample of all spores leads to more rapid adaptation than the transfer of the visually ‘fittest’ sector. The rates of compensatory evolution in the constant and the alternating environment did not differ. Moreover, haploid strains tend to have a higher rate of adaptation than isogenic diploid strains.",
keywords = "deleterious mutations, filamentous fungi, escherichia-coli, evolution, pyrimethanil, populations, fludioxonil, bacteria",
author = "S.E. Schoustra and S.M. Slakhorst-Wandel and A.J.M. Debets and R.F. Hoekstra",
note = "European society for evolutionary biology",
year = "2005",
doi = "10.1111/j.1420-9101.2005.00934.x",
language = "English",
volume = "18",
pages = "771--778",
journal = "Journal of Evolutionary Biology",
issn = "1010-061X",
publisher = "Wiley",
number = "4",

}

Comparing artificial and natural selection in rate of adaptation to genetic stress in Aspergillus nidulans. / Schoustra, S.E.; Slakhorst-Wandel, S.M.; Debets, A.J.M.; Hoekstra, R.F.

In: Journal of Evolutionary Biology, Vol. 18, No. 4, 2005, p. 771-778.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Comparing artificial and natural selection in rate of adaptation to genetic stress in Aspergillus nidulans

AU - Schoustra, S.E.

AU - Slakhorst-Wandel, S.M.

AU - Debets, A.J.M.

AU - Hoekstra, R.F.

N1 - European society for evolutionary biology

PY - 2005

Y1 - 2005

N2 - In an experimental study of adaptation to negative pleiotropic effects of a major fungicide resistance mutation in the filamentous fungus Aspergillus nidulans we have investigated the relative effectiveness of artificial selection vs. natural selection on the rate of compensatory evolution. Using mycelial growth rate as a fitness measure, artificial selection involved the weekly transfer of the fastest growing sector onto a fresh plate. Natural selection was approximated by transferring random samples of all the spores produced by the mycelium. Fungicide resistant and fungicide sensitive haploid and diploid strains were used in an evolution experiment over 10 weekly transfers, which is equivalent to 1200 cell cycles. Two different environmental conditions were applied: a constant fungicide-free environment and a weekly alternation between presence and absence of fungicide. Results show that for all strains and conditions used the transfer of a random sample of all spores leads to more rapid adaptation than the transfer of the visually ‘fittest’ sector. The rates of compensatory evolution in the constant and the alternating environment did not differ. Moreover, haploid strains tend to have a higher rate of adaptation than isogenic diploid strains.

AB - In an experimental study of adaptation to negative pleiotropic effects of a major fungicide resistance mutation in the filamentous fungus Aspergillus nidulans we have investigated the relative effectiveness of artificial selection vs. natural selection on the rate of compensatory evolution. Using mycelial growth rate as a fitness measure, artificial selection involved the weekly transfer of the fastest growing sector onto a fresh plate. Natural selection was approximated by transferring random samples of all the spores produced by the mycelium. Fungicide resistant and fungicide sensitive haploid and diploid strains were used in an evolution experiment over 10 weekly transfers, which is equivalent to 1200 cell cycles. Two different environmental conditions were applied: a constant fungicide-free environment and a weekly alternation between presence and absence of fungicide. Results show that for all strains and conditions used the transfer of a random sample of all spores leads to more rapid adaptation than the transfer of the visually ‘fittest’ sector. The rates of compensatory evolution in the constant and the alternating environment did not differ. Moreover, haploid strains tend to have a higher rate of adaptation than isogenic diploid strains.

KW - deleterious mutations

KW - filamentous fungi

KW - escherichia-coli

KW - evolution

KW - pyrimethanil

KW - populations

KW - fludioxonil

KW - bacteria

U2 - 10.1111/j.1420-9101.2005.00934.x

DO - 10.1111/j.1420-9101.2005.00934.x

M3 - Article

VL - 18

SP - 771

EP - 778

JO - Journal of Evolutionary Biology

JF - Journal of Evolutionary Biology

SN - 1010-061X

IS - 4

ER -