Comparative Proteomics Provides Insights into Metabolic Responses in Rat Liver to Isolated Soy and Meat Proteins

Shangxin Song, G.J.E.J. Hooiveld, W. Zhang, Mengjie Li, Fan Zhoa, Xinglian Xu, M.R. Müller, Chunbao Li*, Guanghong Zhou*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)


It has been reported that isolated dietary soy and meat proteins have distinct effects on physiology and liver gene expression, but the impact on protein expression responses are unknown. Because these may differ from gene expression responses, we investigated dietary protein-induced changes in liver proteome. Rats were fed for 1 week semisynthetic diets that differed only regarding protein source; casein (reference) was fully replaced by isolated soy, chicken, fish, or pork protein. Changes in liver
proteome were measured by iTRAQ labeling and LC−ESI−MS/ MS. A robust set totaling 1437 unique proteins was identified and subjected to differential protein analysis and biological interpretation. Compared with casein, all other protein sources reduced the abundance of proteins involved in fatty acid metabolism and Pparα signaling pathway. All dietary proteins, except chicken, increased oxidoreductive transformation reactions but reduced energy and essential amino acid metabolic pathways. Only soy protein
increased the metabolism of sulfur-containing and nonessential amino acids. Soy and fish proteins increased translation and mRNA processing, whereas only chicken protein increased TCA cycle but reduced immune responses. These findings were partially in line with previously reported transcriptome results. This study further shows the distinct effects of soy and meat proteins on liver metabolism in rats.
Original languageEnglish
Pages (from-to)1135-1142
JournalJournal of Proteome Research
Issue number4
Publication statusPublished - 2016


  • animal protein
  • chicken protein
  • fish protein
  • isolated protein
  • metabolic syndrome
  • molecular nutrition
  • nutrigenomics
  • pork protein
  • proteomics


Dive into the research topics of 'Comparative Proteomics Provides Insights into Metabolic Responses in Rat Liver to Isolated Soy and Meat Proteins'. Together they form a unique fingerprint.

Cite this