Abstract
The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.
Original language | English |
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Article number | e3133 |
Journal | PeerJ |
Volume | 5 |
DOIs | |
Publication status | Published - 30 Mar 2017 |
Keywords
- Amino acid metabolism
- Fumarate
- Nitrogen metabolism
- Proteomics
- Rhizopus delemar
- Transcriptomics
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Rhizopus delemar LC-MS/MS high low oxygen
Odoni, D. I. (Creator) & Martins dos Santos, V. (Creator), Wageningen University, 15 Feb 2017
https://www.ebi.ac.uk/pride/archive/projects/PXD004600
Dataset
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Rhizopus delemar grown under high and low oxygen conditions
Odoni, D. I. (Creator), Tamayo Ramos, J. A. (Creator), Sloothaak, J. (Creator), van Heck, R. G. A. (Creator), Martins dos Santos, V. (Creator), de Graaff, L. H. (Creator), Suarez Diez, M. (Creator) & Schaap, P. (Creator), Wageningen University, 16 Feb 2017
https://www.ebi.ac.uk/ena/data/view/PRJEB14210
Dataset