Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation

Dorett I. Odoni, Juan A. Tamayo-Ramos, Jasper Sloothaak, Ruben van Heck, Vitor A.P. Martins dos Santos, Leo H. de Graaff, Maria Suarez-Diez, Peter J. Schaap

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.

LanguageEnglish
Article numbere3133
JournalPeerJ
Volume5
DOIs
Publication statusPublished - 30 Mar 2017

Fingerprint

Rhizopus
Fumarates
amino acid metabolism
Proteomics
proteomics
Amino Acids
Starvation
Metabolic Networks and Pathways
Fungi
transcriptomics
starvation
Urea
biochemical pathways
fumarates
Nitrogen
urea
fungi
nitrogen

Keywords

  • Amino acid metabolism
  • Fumarate
  • Nitrogen metabolism
  • Proteomics
  • Rhizopus delemar
  • Transcriptomics

Cite this

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title = "Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation",
abstract = "The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.",
keywords = "Amino acid metabolism, Fumarate, Nitrogen metabolism, Proteomics, Rhizopus delemar, Transcriptomics",
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Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation. / Odoni, Dorett I.; Tamayo-Ramos, Juan A.; Sloothaak, Jasper; van Heck, Ruben; Martins dos Santos, Vitor A.P.; de Graaff, Leo H.; Suarez-Diez, Maria; Schaap, Peter J.

In: PeerJ, Vol. 5, e3133, 30.03.2017.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Odoni, Dorett I.

AU - Tamayo-Ramos, Juan A.

AU - Sloothaak, Jasper

AU - van Heck, Ruben

AU - Martins dos Santos, Vitor A.P.

AU - de Graaff, Leo H.

AU - Suarez-Diez, Maria

AU - Schaap, Peter J.

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KW - Nitrogen metabolism

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