Abstract
The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.
| Language | English |
|---|---|
| Article number | e3133 |
| Journal | PeerJ |
| Volume | 5 |
| DOIs | |
| Publication status | Published - 30 Mar 2017 |
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Keywords
- Amino acid metabolism
- Fumarate
- Nitrogen metabolism
- Proteomics
- Rhizopus delemar
- Transcriptomics
Cite this
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Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation. / Odoni, Dorett I.; Tamayo-Ramos, Juan A.; Sloothaak, Jasper; van Heck, Ruben; Martins dos Santos, Vitor A.P.; de Graaff, Leo H.; Suarez-Diez, Maria; Schaap, Peter J.
In: PeerJ, Vol. 5, e3133, 30.03.2017.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation
AU - Odoni, Dorett I.
AU - Tamayo-Ramos, Juan A.
AU - Sloothaak, Jasper
AU - van Heck, Ruben
AU - Martins dos Santos, Vitor A.P.
AU - de Graaff, Leo H.
AU - Suarez-Diez, Maria
AU - Schaap, Peter J.
PY - 2017/3/30
Y1 - 2017/3/30
N2 - The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.
AB - The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.
KW - Amino acid metabolism
KW - Fumarate
KW - Nitrogen metabolism
KW - Proteomics
KW - Rhizopus delemar
KW - Transcriptomics
U2 - 10.7717/peerj.3133
DO - 10.7717/peerj.3133
M3 - Article
VL - 5
JO - PeerJ
T2 - PeerJ
JF - PeerJ
SN - 2167-8359
M1 - e3133
ER -