Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology

N. Mach Casellas; E. Keuning; L. Kruijt; M. Hortós; J. Arnau; M.F.W. te Pas

Abstract

The aim of this study was to identify biomarkers for breed and muscle type through a proteome differential analysis. Samples from the semimembranosus muscle (SM) and longissimus muscle (LM) were collected 24 h post-mortem from 79 male pigs (174±6 d) of the Landrace, Duroc, Large White, Pietrain and Belgian Landrace breeds, following a 2 x 5 factorial design. The Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry analysis was performed using the Protein Chip System Series 4000 equipment. The CM10 ProteinChip® array type and binding buffer pH5 containing 0.1% Triton was chosen. Data was normalized and corrected for background. Peak intensity was log-transformed. The number of peaks (36.33±0.87) was not affected by breed and muscle type. However, the average intensity of peaks was affected (P

Original language	English
Title of host publication	Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009
Publisher	Wageningen Academic Publishers
Pages	34-34
ISBN (Print)	9789086861217
Publication status	Published - 2009
Event	60th Annual Meeting of the European Association for Animal Production - Duration: 24 Aug 2009 → 27 Aug 2009

Conference/symposium

Conference/symposium	60th Annual Meeting of the European Association for Animal Production
Period	24/08/09 → 27/08/09

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https://edepot.wur.nl/135107

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Mach Casellas, N., Keuning, E., Kruijt, L., Hortós, M., Arnau, J., & te Pas, M. F. W. (2009). Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology. In Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009 (pp. 34-34). Wageningen Academic Publishers. https://edepot.wur.nl/135107

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title = "Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology",

abstract = "The aim of this study was to identify biomarkers for breed and muscle type through a proteome differential analysis. Samples from the semimembranosus muscle (SM) and longissimus muscle (LM) were collected 24 h post-mortem from 79 male pigs (174±6 d) of the Landrace, Duroc, Large White, Pietrain and Belgian Landrace breeds, following a 2 x 5 factorial design. The Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry analysis was performed using the Protein Chip System Series 4000 equipment. The CM10 ProteinChip{\textregistered} array type and binding buffer pH5 containing 0.1\% Triton was chosen. Data was normalized and corrected for background. Peak intensity was log-transformed. The number of peaks (36.33±0.87) was not affected by breed and muscle type. However, the average intensity of peaks was affected (P",

author = "\{Mach Casellas\}, N. and E. Keuning and L. Kruijt and M. Hort{\'o}s and J. Arnau and \{te Pas\}, M.F.W.",

year = "2009",

language = "English",

isbn = "9789086861217",

pages = "34--34",

booktitle = "Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009",

publisher = "Wageningen Academic Publishers",

address = "Netherlands",

note = "60th Annual Meeting of the European Association for Animal Production ; Conference date: 24-08-2009 Through 27-08-2009",

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Mach Casellas, N, Keuning, E, Kruijt, L, Hortós, M, Arnau, J & te Pas, MFW 2009, Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology. in Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009. Wageningen Academic Publishers, pp. 34-34, 60th Annual Meeting of the European Association for Animal Production, 24/08/09. <https://edepot.wur.nl/135107>

Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology. / Mach Casellas, N.; Keuning, E.; Kruijt, L. et al.
Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009. Wageningen Academic Publishers, 2009. p. 34-34.

Research output: Chapter in Book/Report/Conference proceeding › Abstract

TY - CHAP

T1 - Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology

AU - Mach Casellas, N.

AU - Keuning, E.

AU - Kruijt, L.

AU - Hortós, M.

AU - Arnau, J.

AU - te Pas, M.F.W.

PY - 2009

Y1 - 2009

N2 - The aim of this study was to identify biomarkers for breed and muscle type through a proteome differential analysis. Samples from the semimembranosus muscle (SM) and longissimus muscle (LM) were collected 24 h post-mortem from 79 male pigs (174±6 d) of the Landrace, Duroc, Large White, Pietrain and Belgian Landrace breeds, following a 2 x 5 factorial design. The Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry analysis was performed using the Protein Chip System Series 4000 equipment. The CM10 ProteinChip® array type and binding buffer pH5 containing 0.1% Triton was chosen. Data was normalized and corrected for background. Peak intensity was log-transformed. The number of peaks (36.33±0.87) was not affected by breed and muscle type. However, the average intensity of peaks was affected (P

AB - The aim of this study was to identify biomarkers for breed and muscle type through a proteome differential analysis. Samples from the semimembranosus muscle (SM) and longissimus muscle (LM) were collected 24 h post-mortem from 79 male pigs (174±6 d) of the Landrace, Duroc, Large White, Pietrain and Belgian Landrace breeds, following a 2 x 5 factorial design. The Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry analysis was performed using the Protein Chip System Series 4000 equipment. The CM10 ProteinChip® array type and binding buffer pH5 containing 0.1% Triton was chosen. Data was normalized and corrected for background. Peak intensity was log-transformed. The number of peaks (36.33±0.87) was not affected by breed and muscle type. However, the average intensity of peaks was affected (P

M3 - Abstract

SN - 9789086861217

SP - 34

EP - 34

BT - Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009

PB - Wageningen Academic Publishers

T2 - 60th Annual Meeting of the European Association for Animal Production

Y2 - 24 August 2009 through 27 August 2009

ER -

Mach Casellas N, Keuning E, Kruijt L, Hortós M, Arnau J, te Pas MFW. Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology. In Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009. Wageningen Academic Publishers. 2009. p. 34-34

Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology

Abstract

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