Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology

N. Mach Casellas, E. Keuning, L. Kruijt, M. Hortós, J. Arnau, M.F.W. te Pas

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

The aim of this study was to identify biomarkers for breed and muscle type through a proteome differential analysis. Samples from the semimembranosus muscle (SM) and longissimus muscle (LM) were collected 24 h post-mortem from 79 male pigs (174±6 d) of the Landrace, Duroc, Large White, Pietrain and Belgian Landrace breeds, following a 2 x 5 factorial design. The Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry analysis was performed using the Protein Chip System Series 4000 equipment. The CM10 ProteinChip® array type and binding buffer pH5 containing 0.1% Triton was chosen. Data was normalized and corrected for background. Peak intensity was log-transformed. The number of peaks (36.33±0.87) was not affected by breed and muscle type. However, the average intensity of peaks was affected (P
Original languageEnglish
Title of host publicationBook of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009
PublisherWageningen Academic Publishers
Pages34-34
ISBN (Print)9789086861217
Publication statusPublished - 2009
Event60th Annual Meeting of the European Association for Animal Production -
Duration: 24 Aug 200927 Aug 2009

Conference

Conference60th Annual Meeting of the European Association for Animal Production
Period24/08/0927/08/09

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    Mach Casellas, N., Keuning, E., Kruijt, L., Hortós, M., Arnau, J., & te Pas, M. F. W. (2009). Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology. In Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009 (pp. 34-34). Wageningen Academic Publishers.