Comparative proteome approach demonstrates that platelet-derived growth factor C and D efficiently induce proliferation while maintaining multipotency of hMSCs.

A.M. Sotoca, J. Roelofs-Hendriks, S. Boeren, P.M. van der Kraan, J. Vervoort, E.J. van Zoelen, E. Piek

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

This is the first study that comprehensively describes the effects of the platelet-derived growth factor (PDGF) isoforms C and D during in vitro expansion of human mesenchymal stem cells (hMSCs). Our results show that PDGFs can enhance proliferation of hMSCs without affecting their multipotency. It is of great value to culture and expand hMSCs in a safe and effective manner without losing their multipotency for manipulation and further development of cell-based therapies. Moreover, differential effects of PDGF isoforms have been observed on lineage-specific differentiation induced by BMP2 and Vitamin D3. Based on label-free LC-based quantitative proteomics approach we have furthermore identified specific pathways induced by PDGFs during the proliferation process, showing the importance of bioinformatics tools to study cell function.
Original languageEnglish
Pages (from-to)2649-2662
JournalExperimental Cell Research
Volume319
Issue number17
DOIs
Publication statusPublished - 2013

Keywords

  • mesenchymal stem-cells
  • nectin-like molecules
  • bone-marrow
  • malignant-transformation
  • quantitative proteomics
  • adhesion molecules
  • factor receptors
  • adherent cells
  • in-vitro
  • expansion

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