comparative pathogenesis of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in noctuid hosts of different susceptibility

A. Herz, R.G. Kleespies, J. Huber, Chen Xinwen, J.M. Vlak

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

A new cell line was established from the embryos of the insect Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). The cell line contains several morphologically different cell types and was distinguished from three other lepidopteran cell lines propagated in the laboratory by DNA amplification fingerprinting. The cultured cells, which we officially named WU-CcE-1 cells, were permissive for infection by C. chalcites nucleopolyhedrovirus (ChchNPV) and large numbers of occlusion bodies were produced that retained their infectivity for C. chalcites larvae. The CcE-1 cells were also permissive for Trichoplusia ni single nucleopolyhedrovirus (TnSNPV). ChchNPV could be passaged in these cells for at least four passages indicating that budded virus production was supported. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Helicoverpa armigera (Hear) NPV both induced apoptosis in these cells. The results obtained indicate that the CcE-1 cell line will be a useful tool in the study of both ChchNPV and TnSNPV.
Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalJournal of Invertebrate Pathology
Volume83
DOIs
Publication statusPublished - 2003

Keywords

  • nuclear polyhedrosis-virus
  • heliothis-virescens
  • trichoplusia-ni
  • lepidoptera
  • larvae
  • baculoviruses
  • activation
  • infection
  • replication
  • resistance

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