Comparative genomics of plant pathogenic Botrytis species with distinct host specificity

Claudio A. Valero-Jiménez, Javier Veloso, Martijn Staats, Jan A.L. van Kan*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Background: Fungi of the genus Botrytis (presently containing ~ 35 species) are able to infect more than 1400 different plant species and cause losses in a wide range of crops of economic importance. The best studied species is B. cinerea, which has a broad host range and is one of the best studied necrotrophic plant pathogenic fungi. Most other Botrytis spp. have a narrow host range and have been studied in less detail. To characterize genomic variation among different representatives of Botrytis spp., we sequenced and annotated the draft genomes of nine Botrytis species: B. calthae, B. convoluta, B. elliptica, B. galanthina, B. hyacinthi, B. narcissicola, B. paeoniae, B. porri and B. tulipae. Results: Bioinformatics and comparative genomics tools were applied to determine a core of 7668 shared protein families in all Botrytis species, which grouped them in two distinct phylogenetic clades. The secretome of all nine Botrytis spp. was similar in number (ranging from 716 to 784 predicted proteins). A detailed analysis of the molecular functions of the secretome revealed that shared activities were highly similar. Orthologs to effectors functionally studied in B. cinerea were also present in the other Botrytis species. A complex pattern of presence/absence of secondary metabolite biosynthetic key enzymes was observed. Conclusions: Comparative genomics of Botrytis show that overall, species share the main signatures and protein families in the secreted proteins, and of known effectors. Our study provides leads to study host range determinants in the genus Botrytis and provides a stepping stone to elucidate the roles of effector candidates in the infection process of these species.

Original languageEnglish
Article number203
JournalBMC Genomics
Volume20
Issue number1
DOIs
Publication statusPublished - 12 Mar 2019

Keywords

  • Effector
  • Grey mould
  • Necrotroph
  • Secondary metabolite
  • Secretome

Fingerprint Dive into the research topics of 'Comparative genomics of plant pathogenic Botrytis species with distinct host specificity'. Together they form a unique fingerprint.

  • Datasets

    Draft genomes of nine plant pathogenic Botrytis species

    Valero Jimenez, C. (Creator), Veloso, J. (Creator), Staats, M. (Creator) & van Kan, J. (Creator), Wageningen University, 5 Sep 2017

    Dataset

    Cite this