Comparative genomics of campylobacter iguaniorum to unravel genetic regions associated with reptilian hosts

Maarten J. Gilbert, William G. Miller, Emma Yee, Marja Kik, Aldert L. Zomer, Jaap A. Wagenaar, Birgitta Duim

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Campylobacter iguaniorum is most closely related to the species C. fetus, C. hyointestinalis, andC. lanienae. Reptiles, chelonians and lizards in particular, appear to be a primary reservoir of this Campylobacter species. Here we report the genome comparison of C. iguaniorumstrain 1485E, isolated from a bearded dragon (Pogona vitticeps), and strain 2463D, isolated froma green iguana (Iguana iguana), with the genomes of closely related taxa, in particular with reptile-Associated C. fetus subsp.Testudinum. In contrast to C. fetus, C. iguaniorum is lacking an S-layer encoding region. Furthermore, a defined lipooligosaccharide biosynthesis locus, encoding multiple glycosyltransferases and bounded by waa genes, is absent from C. iguaniorum. Instead, multiple predicted glycosylation regionswere identified inC. iguaniorum.One of these regions is>50 kb withdeviantG+Ccontent, suggesting acquisition via lateral transfer. These similar, but non-homologous glycosylation regions were located at the same position on the genome in both strains. Multiple genes encoding respiratory enzymes not identified to date within the C. fetus clade were present. C. iguaniorum shared highest homology with C. hyointestinalis and C. fetus. As in reptile-Associated C. fetus subsp.Testudinum, a putative tricarballylate catabolism locus was identified. However, despite colonizing a shared host, no recent recombination between both taxa was detected. This genomic study provides a better understanding of host adaptation, virulence, phylogeny, and evolution of C. iguaniorum and related Campylobacter taxa.

Original languageEnglish
Pages (from-to)3022-3029
JournalGenome Biology and Evolution
Volume8
Issue number9
DOIs
Publication statusPublished - 2016

Fingerprint

Campylobacter
Genomics
reptile
fetus
Iguana iguana
genomics
Fetus
genome
Iguanas
Reptiles
reptiles
glycosylation
catabolism
gene
Genome
Campylobacter hyointestinalis
virulence
homology
Glycosylation
lizard

Keywords

  • Campylobacter Iguaniorum
  • Comparative Genomics
  • Evolution
  • Phylogeny
  • Recombination
  • Reptile

Cite this

Gilbert, M. J., Miller, W. G., Yee, E., Kik, M., Zomer, A. L., Wagenaar, J. A., & Duim, B. (2016). Comparative genomics of campylobacter iguaniorum to unravel genetic regions associated with reptilian hosts. Genome Biology and Evolution, 8(9), 3022-3029. https://doi.org/10.1093/gbe/evw218
Gilbert, Maarten J. ; Miller, William G. ; Yee, Emma ; Kik, Marja ; Zomer, Aldert L. ; Wagenaar, Jaap A. ; Duim, Birgitta. / Comparative genomics of campylobacter iguaniorum to unravel genetic regions associated with reptilian hosts. In: Genome Biology and Evolution. 2016 ; Vol. 8, No. 9. pp. 3022-3029.
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abstract = "Campylobacter iguaniorum is most closely related to the species C. fetus, C. hyointestinalis, andC. lanienae. Reptiles, chelonians and lizards in particular, appear to be a primary reservoir of this Campylobacter species. Here we report the genome comparison of C. iguaniorumstrain 1485E, isolated from a bearded dragon (Pogona vitticeps), and strain 2463D, isolated froma green iguana (Iguana iguana), with the genomes of closely related taxa, in particular with reptile-Associated C. fetus subsp.Testudinum. In contrast to C. fetus, C. iguaniorum is lacking an S-layer encoding region. Furthermore, a defined lipooligosaccharide biosynthesis locus, encoding multiple glycosyltransferases and bounded by waa genes, is absent from C. iguaniorum. Instead, multiple predicted glycosylation regionswere identified inC. iguaniorum.One of these regions is>50 kb withdeviantG+Ccontent, suggesting acquisition via lateral transfer. These similar, but non-homologous glycosylation regions were located at the same position on the genome in both strains. Multiple genes encoding respiratory enzymes not identified to date within the C. fetus clade were present. C. iguaniorum shared highest homology with C. hyointestinalis and C. fetus. As in reptile-Associated C. fetus subsp.Testudinum, a putative tricarballylate catabolism locus was identified. However, despite colonizing a shared host, no recent recombination between both taxa was detected. This genomic study provides a better understanding of host adaptation, virulence, phylogeny, and evolution of C. iguaniorum and related Campylobacter taxa.",
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Gilbert, MJ, Miller, WG, Yee, E, Kik, M, Zomer, AL, Wagenaar, JA & Duim, B 2016, 'Comparative genomics of campylobacter iguaniorum to unravel genetic regions associated with reptilian hosts' Genome Biology and Evolution, vol. 8, no. 9, pp. 3022-3029. https://doi.org/10.1093/gbe/evw218

Comparative genomics of campylobacter iguaniorum to unravel genetic regions associated with reptilian hosts. / Gilbert, Maarten J.; Miller, William G.; Yee, Emma; Kik, Marja; Zomer, Aldert L.; Wagenaar, Jaap A.; Duim, Birgitta.

In: Genome Biology and Evolution, Vol. 8, No. 9, 2016, p. 3022-3029.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Gilbert, Maarten J.

AU - Miller, William G.

AU - Yee, Emma

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