Comparative evaluation of six ELISA's for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus.

E. Brocchi, I.E. Bergmann, A. Dekker, D.J. Paton, D.J. Sammin, M. Greiner, S. Graziola, F. De Simone, H. Yadin, B. Haas, N. Bulut, V. Malirat, E. Neitzert, N. Goris, S. Parida, K. Sorensen, K. De Clerq

    Research output: Contribution to journalArticleAcademicpeer-review

    146 Citations (Scopus)

    Abstract

    To validate the use of serology in substantiating freedom from infection after foot-and-mouth disease (FMD) outbreaks have been controlled by measures that include vaccination, 3551 sera were tested with six assays that detect antibodies to the non-structural proteins of FMD virus. The sera came from naïve, vaccinated, infected and vaccinated-and-infected animals; two-thirds from cattle, the remainder from sheep and pigs. The assays were covariant for sensitivity, but not necessarily for specificity. A commercial kit from Cedi-diagnostics and an in-house assay from IZS-Brescia were comparable to the NCPanaftosa-screening index method described in the Diagnostic Manual of the World Animal Health Organisation. Using these three tests the specificity and sensitivity for the detection of carriers in vaccinated cattle approaches or exceeds 99% and 90%, respectively.
    Original languageEnglish
    Pages (from-to)6966-6979
    JournalVaccine
    Volume24
    Issue number47-48
    DOIs
    Publication statusPublished - 2006

    Keywords

    • differentiating infection
    • vaccinated cattle
    • polyprotein 3abc
    • recombinant
    • antigen
    • baculovirus
    • strategy
    • animals
    • tests
    • assay

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