Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research

Wouter Graumans, Michelle Schinkel, Geert-Jan van Gemert, Jeroen Spitzen, Teun Bousema*, Pascal Miesen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Background
Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system.
Methods
Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibed blood meal volume and Plasmodium falciparum infection intensity on mosquito guts.
Results
While mosquito feeding rate and blood meal volume were comparable between Hemotek and glass feeders, a loss in transmission was observed using the Hemotek feeder with a conventional collagen membrane. There was no difference in transmission between both feeders when Parafilm was used as the membrane.
Conclusions
Hemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research.
Original languageEnglish
Article numbertrac135
Pages (from-to)476-478
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume117
Issue number6
Early online date13 Jan 2023
DOIs
Publication statusPublished - 2 Jun 2023

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