Comparative analysis of Bacillus weihenstephanensis KBAB4 spores obtained at different temperatures

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27 Citations (Scopus)

Abstract

The impact of Bacillus weihenstephanensis KBAB4 sporulation temperature history was assessed on spore heat resistance, germination and outgrowth capacity at a temperature range from 7 to 30 °C. Sporulation rate and efficiency decreased at low temperature, as cells sporulated at 12, 20 and 30 °C with approximately 99% efficiency, whereas at 7 °C and 10 °C, a maximum 15% of sporulation was reached. Spores formed at 30 °C showed the highest wet heat resistance at 95 °C, with spores formed at 7 and 10 °C displaying only survival of 15 min exposure at 70 °C, indicating their low level heat resistance. RT-PCR analysis revealed expression of sporulation sigma factor sigG, and germinant receptor operons gerI, gerK, gerL, gerR, gerS, and (plasmid-located) gerS2 to be activated in all sporulation conditions tested. Subsequent germination assays revealed a combination of inosine and L-Alanine to be very efficient, triggering over 99% of the spores to germinate, with spores obtained at 30 °C showing the highest germination rates (99%). Notably, spores obtained at 12, 20 and 30 °C, germinated at all tested temperatures, showing > 70% spore germination even at temperatures as low as 5 °C. Less than 5% of spores obtained at 7 and 10 °C showed a germination response. Furthermore, spores produced at 12, 20 and 30 °C showed similar outgrowth effiency at these temperatures, indicating that low temperature sporulation history does not improve low temperature outgrowth performance. Insights obtained in sporulation and germination behaviour of B. weihenstephanensis KBAB4, in combination with the availability of its genome sequence, may contribute to our understanding of the behaviour of psychrotolerant spoilage and pathogenic Bacilli
LanguageEnglish
Pages146-153
JournalInternational Journal of Food Microbiology
Volume140
Issue number2-3
DOIs
Publication statusPublished - 2010

Fingerprint

Bacillus weihenstephanensis
Spores
Bacillus
sporulation
spores
Germination
Temperature
germination
temperature
Hot Temperature
heat tolerance
sigma factors
history
Sigma Factor
Inosine
spore germination
operon
spoilage
alanine
Operon

Keywords

  • sporulation temperature
  • cereus atcc-14579
  • dipicolinic acid
  • subtilis spores
  • l-alanine
  • germination
  • heat
  • resistance
  • strains
  • inactivation

Cite this

@article{7256719766d5429fa5379b576ba4cdc3,
title = "Comparative analysis of Bacillus weihenstephanensis KBAB4 spores obtained at different temperatures",
abstract = "The impact of Bacillus weihenstephanensis KBAB4 sporulation temperature history was assessed on spore heat resistance, germination and outgrowth capacity at a temperature range from 7 to 30 °C. Sporulation rate and efficiency decreased at low temperature, as cells sporulated at 12, 20 and 30 °C with approximately 99{\%} efficiency, whereas at 7 °C and 10 °C, a maximum 15{\%} of sporulation was reached. Spores formed at 30 °C showed the highest wet heat resistance at 95 °C, with spores formed at 7 and 10 °C displaying only survival of 15 min exposure at 70 °C, indicating their low level heat resistance. RT-PCR analysis revealed expression of sporulation sigma factor sigG, and germinant receptor operons gerI, gerK, gerL, gerR, gerS, and (plasmid-located) gerS2 to be activated in all sporulation conditions tested. Subsequent germination assays revealed a combination of inosine and L-Alanine to be very efficient, triggering over 99{\%} of the spores to germinate, with spores obtained at 30 °C showing the highest germination rates (99{\%}). Notably, spores obtained at 12, 20 and 30 °C, germinated at all tested temperatures, showing > 70{\%} spore germination even at temperatures as low as 5 °C. Less than 5{\%} of spores obtained at 7 and 10 °C showed a germination response. Furthermore, spores produced at 12, 20 and 30 °C showed similar outgrowth effiency at these temperatures, indicating that low temperature sporulation history does not improve low temperature outgrowth performance. Insights obtained in sporulation and germination behaviour of B. weihenstephanensis KBAB4, in combination with the availability of its genome sequence, may contribute to our understanding of the behaviour of psychrotolerant spoilage and pathogenic Bacilli",
keywords = "sporulation temperature, cereus atcc-14579, dipicolinic acid, subtilis spores, l-alanine, germination, heat, resistance, strains, inactivation",
author = "D. Garcia and {van der Voort}, M. and T. Abee",
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pages = "146--153",
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Comparative analysis of Bacillus weihenstephanensis KBAB4 spores obtained at different temperatures. / Garcia, D.; van der Voort, M.; Abee, T.

In: International Journal of Food Microbiology, Vol. 140, No. 2-3, 2010, p. 146-153.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Comparative analysis of Bacillus weihenstephanensis KBAB4 spores obtained at different temperatures

AU - Garcia, D.

AU - van der Voort, M.

AU - Abee, T.

PY - 2010

Y1 - 2010

N2 - The impact of Bacillus weihenstephanensis KBAB4 sporulation temperature history was assessed on spore heat resistance, germination and outgrowth capacity at a temperature range from 7 to 30 °C. Sporulation rate and efficiency decreased at low temperature, as cells sporulated at 12, 20 and 30 °C with approximately 99% efficiency, whereas at 7 °C and 10 °C, a maximum 15% of sporulation was reached. Spores formed at 30 °C showed the highest wet heat resistance at 95 °C, with spores formed at 7 and 10 °C displaying only survival of 15 min exposure at 70 °C, indicating their low level heat resistance. RT-PCR analysis revealed expression of sporulation sigma factor sigG, and germinant receptor operons gerI, gerK, gerL, gerR, gerS, and (plasmid-located) gerS2 to be activated in all sporulation conditions tested. Subsequent germination assays revealed a combination of inosine and L-Alanine to be very efficient, triggering over 99% of the spores to germinate, with spores obtained at 30 °C showing the highest germination rates (99%). Notably, spores obtained at 12, 20 and 30 °C, germinated at all tested temperatures, showing > 70% spore germination even at temperatures as low as 5 °C. Less than 5% of spores obtained at 7 and 10 °C showed a germination response. Furthermore, spores produced at 12, 20 and 30 °C showed similar outgrowth effiency at these temperatures, indicating that low temperature sporulation history does not improve low temperature outgrowth performance. Insights obtained in sporulation and germination behaviour of B. weihenstephanensis KBAB4, in combination with the availability of its genome sequence, may contribute to our understanding of the behaviour of psychrotolerant spoilage and pathogenic Bacilli

AB - The impact of Bacillus weihenstephanensis KBAB4 sporulation temperature history was assessed on spore heat resistance, germination and outgrowth capacity at a temperature range from 7 to 30 °C. Sporulation rate and efficiency decreased at low temperature, as cells sporulated at 12, 20 and 30 °C with approximately 99% efficiency, whereas at 7 °C and 10 °C, a maximum 15% of sporulation was reached. Spores formed at 30 °C showed the highest wet heat resistance at 95 °C, with spores formed at 7 and 10 °C displaying only survival of 15 min exposure at 70 °C, indicating their low level heat resistance. RT-PCR analysis revealed expression of sporulation sigma factor sigG, and germinant receptor operons gerI, gerK, gerL, gerR, gerS, and (plasmid-located) gerS2 to be activated in all sporulation conditions tested. Subsequent germination assays revealed a combination of inosine and L-Alanine to be very efficient, triggering over 99% of the spores to germinate, with spores obtained at 30 °C showing the highest germination rates (99%). Notably, spores obtained at 12, 20 and 30 °C, germinated at all tested temperatures, showing > 70% spore germination even at temperatures as low as 5 °C. Less than 5% of spores obtained at 7 and 10 °C showed a germination response. Furthermore, spores produced at 12, 20 and 30 °C showed similar outgrowth effiency at these temperatures, indicating that low temperature sporulation history does not improve low temperature outgrowth performance. Insights obtained in sporulation and germination behaviour of B. weihenstephanensis KBAB4, in combination with the availability of its genome sequence, may contribute to our understanding of the behaviour of psychrotolerant spoilage and pathogenic Bacilli

KW - sporulation temperature

KW - cereus atcc-14579

KW - dipicolinic acid

KW - subtilis spores

KW - l-alanine

KW - germination

KW - heat

KW - resistance

KW - strains

KW - inactivation

U2 - 10.1016/j.ijfoodmicro.2010.04.005

DO - 10.1016/j.ijfoodmicro.2010.04.005

M3 - Article

VL - 140

SP - 146

EP - 153

JO - International Journal of Food Microbiology

T2 - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

IS - 2-3

ER -