Comparative AFLP reveals paternal sex ratio chromosome specific DNA sequences in the parasitoid wasp Trichogramma kaykai

J.J.F.A. van Vugt, R.G.M. van der Hulst, A. Pruijssers, P.G.H. Verbaarschot, R. Stouthamer, H. de Jong

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The parasitoid wasp Trichogramma kaykai with a haplo-diploid sex determination has a B chromosome called the paternal sex ratio (PSR) chromosome that confers paternal genome loss during early embryogenesis, resulting in male offspring. So far, it is not well known whether the PSR chromosome has unique DNA sequence characteristics. By comparative AFLP fingerprinting of genomic DNA from wasps with and without the PSR chromosome, we isolated DNA from PSR-specific bands. Fourteen of such DNA fragments were analysed to confirm their PSR specificity. Seven were sequenced and two (PT-AFLP 1 and PT-AFLP1 3) were identified as parts of retrotransposon genes based on BLAST searches. Internal primers designed from a third AFLP fragment allowed PCR amplification of a PSR chromosome specific marker, which can be used to screen for the PSR trait in male wasps. Southern analysis revealed a dispersed repetitive nature of this third sequence in the T. kaykai genome, suggesting that it is part of a transposon. A fourth AFLP fragment (PT-AFLP 5) appears to be a large repetitive sequence on the PSR chromosome. This sequence is also found in the genome of both T. kaykai and the closely related species Trichogramma deion, but its distribution on the PSR chromosome strongly resembles that of T. deion rather than that of T. kaykai. Our results provide further insight into the repetitive nature of sequences comprising B chromosomes and their similarities with their host and closely related species
Original languageEnglish
Pages (from-to)447-455
JournalGenome
Volume52
Issue number5
DOIs
Publication statusPublished - 2009

Fingerprint

Wasps
Sex Chromosomes
Sex Ratio
Chromosomes, Human, 4-5
Nucleic Acid Repetitive Sequences
Genome
Retroelements
DNA Fingerprinting
DNA
Diploidy
Genetic Markers
Embryonic Development
Polymerase Chain Reaction

Keywords

  • selfish b-chromosome
  • rye secale-cereale
  • nasonia-vitripennis
  • molecular characterization
  • crepis-capillaris
  • bombyx-mori
  • psr
  • retrotransposon
  • transmission
  • elements

Cite this

van Vugt, J.J.F.A. ; van der Hulst, R.G.M. ; Pruijssers, A. ; Verbaarschot, P.G.H. ; Stouthamer, R. ; de Jong, H. / Comparative AFLP reveals paternal sex ratio chromosome specific DNA sequences in the parasitoid wasp Trichogramma kaykai. In: Genome. 2009 ; Vol. 52, No. 5. pp. 447-455.
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title = "Comparative AFLP reveals paternal sex ratio chromosome specific DNA sequences in the parasitoid wasp Trichogramma kaykai",
abstract = "The parasitoid wasp Trichogramma kaykai with a haplo-diploid sex determination has a B chromosome called the paternal sex ratio (PSR) chromosome that confers paternal genome loss during early embryogenesis, resulting in male offspring. So far, it is not well known whether the PSR chromosome has unique DNA sequence characteristics. By comparative AFLP fingerprinting of genomic DNA from wasps with and without the PSR chromosome, we isolated DNA from PSR-specific bands. Fourteen of such DNA fragments were analysed to confirm their PSR specificity. Seven were sequenced and two (PT-AFLP 1 and PT-AFLP1 3) were identified as parts of retrotransposon genes based on BLAST searches. Internal primers designed from a third AFLP fragment allowed PCR amplification of a PSR chromosome specific marker, which can be used to screen for the PSR trait in male wasps. Southern analysis revealed a dispersed repetitive nature of this third sequence in the T. kaykai genome, suggesting that it is part of a transposon. A fourth AFLP fragment (PT-AFLP 5) appears to be a large repetitive sequence on the PSR chromosome. This sequence is also found in the genome of both T. kaykai and the closely related species Trichogramma deion, but its distribution on the PSR chromosome strongly resembles that of T. deion rather than that of T. kaykai. Our results provide further insight into the repetitive nature of sequences comprising B chromosomes and their similarities with their host and closely related species",
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Comparative AFLP reveals paternal sex ratio chromosome specific DNA sequences in the parasitoid wasp Trichogramma kaykai. / van Vugt, J.J.F.A.; van der Hulst, R.G.M.; Pruijssers, A.; Verbaarschot, P.G.H.; Stouthamer, R.; de Jong, H.

In: Genome, Vol. 52, No. 5, 2009, p. 447-455.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Comparative AFLP reveals paternal sex ratio chromosome specific DNA sequences in the parasitoid wasp Trichogramma kaykai

AU - van Vugt, J.J.F.A.

AU - van der Hulst, R.G.M.

AU - Pruijssers, A.

AU - Verbaarschot, P.G.H.

AU - Stouthamer, R.

AU - de Jong, H.

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AB - The parasitoid wasp Trichogramma kaykai with a haplo-diploid sex determination has a B chromosome called the paternal sex ratio (PSR) chromosome that confers paternal genome loss during early embryogenesis, resulting in male offspring. So far, it is not well known whether the PSR chromosome has unique DNA sequence characteristics. By comparative AFLP fingerprinting of genomic DNA from wasps with and without the PSR chromosome, we isolated DNA from PSR-specific bands. Fourteen of such DNA fragments were analysed to confirm their PSR specificity. Seven were sequenced and two (PT-AFLP 1 and PT-AFLP1 3) were identified as parts of retrotransposon genes based on BLAST searches. Internal primers designed from a third AFLP fragment allowed PCR amplification of a PSR chromosome specific marker, which can be used to screen for the PSR trait in male wasps. Southern analysis revealed a dispersed repetitive nature of this third sequence in the T. kaykai genome, suggesting that it is part of a transposon. A fourth AFLP fragment (PT-AFLP 5) appears to be a large repetitive sequence on the PSR chromosome. This sequence is also found in the genome of both T. kaykai and the closely related species Trichogramma deion, but its distribution on the PSR chromosome strongly resembles that of T. deion rather than that of T. kaykai. Our results provide further insight into the repetitive nature of sequences comprising B chromosomes and their similarities with their host and closely related species

KW - selfish b-chromosome

KW - rye secale-cereale

KW - nasonia-vitripennis

KW - molecular characterization

KW - crepis-capillaris

KW - bombyx-mori

KW - psr

KW - retrotransposon

KW - transmission

KW - elements

U2 - 10.1139/G09-024

DO - 10.1139/G09-024

M3 - Article

VL - 52

SP - 447

EP - 455

JO - Genome

JF - Genome

SN - 0831-2796

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