Combining electroless ionisation mass spectrometry with solid-phase extraction for the direct analysis of beta-agonists in bovine urine

Sjors Rasker; Joris Schipperheijn; Stefan Kooij; Marco H. Blokland; Cees J.M. van Rijn; Ane Arrizabalaga-Larrañaga

doi:10.1007/s00216-025-06019-3

Combining electroless ionisation mass spectrometry with solid-phase extraction for the direct analysis of beta-agonists in bovine urine

Sjors Rasker^*, Joris Schipperheijn, Stefan Kooij, Marco H. Blokland, Cees J.M. van Rijn, Ane Arrizabalaga-Larrañaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A novel method combining electroless ionisation mass spectrometry (ELI-MS) with solid-phase extraction (SPE) cartridges enables direct analysis of beta-agonist residues in bovine urine (SPE-ELI-MS). Using a syringe plunger and a battery-powered DIY syringe pump mounted on an XYZ-linear stage, rapid sample clean-up and precise positioning were achieved. Optimisation of spray solvent and nozzle placement improved performance, yielding high sensitivity, repeatability, linearity, and trueness with internal standard correction. This approach offers a simple, efficient solution for analysing organic residues in biological samples, with strong potential for future on-site applications.

Original language	English
Pages (from-to)	5019-5025
Journal	Analytical and Bioanalytical Chemistry
Volume	417
Issue number	22
Early online date	26 Jul 2025
DOIs	https://doi.org/10.1007/s00216-025-06019-3
Publication status	Published - 2025

Keywords

Ambient ionisation mass spectrometry
Beta-agonists
Bovine urine
Electroless ionisation
ELI-MS
Solid-phase extraction

Access to Document

10.1007/s00216-025-06019-3Licence: CC BY

https://edepot.wur.nl/699054Licence: CC BY

Combining electroless ionisation mass spectrometry with solid-phase extraction for the direct analysis of beta-agonists in bovine urine
Rasker, S. (Creator) & Schipperheijn, J. (Creator), Wageningen Food Safety Research, 6 Feb 2025
DOI: 10.5281/zenodo.14823134
Dataset

Speerpunt 2 - Detecteren (KB-56-002-007, KB-37-002-037)
Blokland, M. (Project Leader)
1/01/22 → 31/12/25
Project: LVVN project

Cite this

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title = "Combining electroless ionisation mass spectrometry with solid-phase extraction for the direct analysis of beta-agonists in bovine urine",

abstract = "A novel method combining electroless ionisation mass spectrometry (ELI-MS) with solid-phase extraction (SPE) cartridges enables direct analysis of beta-agonist residues in bovine urine (SPE-ELI-MS). Using a syringe plunger and a battery-powered DIY syringe pump mounted on an XYZ-linear stage, rapid sample clean-up and precise positioning were achieved. Optimisation of spray solvent and nozzle placement improved performance, yielding high sensitivity, repeatability, linearity, and trueness with internal standard correction. This approach offers a simple, efficient solution for analysing organic residues in biological samples, with strong potential for future on-site applications.",

keywords = "Ambient ionisation mass spectrometry, Beta-agonists, Bovine urine, Electroless ionisation, ELI-MS, Solid-phase extraction",

author = "Sjors Rasker and Joris Schipperheijn and Stefan Kooij and Blokland, \{Marco H.\} and \{van Rijn\}, \{Cees J.M.\} and Ane Arrizabalaga-Larra{\~n}aga",

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doi = "10.1007/s00216-025-06019-3",

language = "English",

volume = "417",

pages = "5019--5025",

journal = "Analytical and Bioanalytical Chemistry",

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T1 - Combining electroless ionisation mass spectrometry with solid-phase extraction for the direct analysis of beta-agonists in bovine urine

AU - Rasker, Sjors

AU - Schipperheijn, Joris

AU - Kooij, Stefan

AU - Blokland, Marco H.

AU - van Rijn, Cees J.M.

AU - Arrizabalaga-Larrañaga, Ane

PY - 2025

Y1 - 2025

N2 - A novel method combining electroless ionisation mass spectrometry (ELI-MS) with solid-phase extraction (SPE) cartridges enables direct analysis of beta-agonist residues in bovine urine (SPE-ELI-MS). Using a syringe plunger and a battery-powered DIY syringe pump mounted on an XYZ-linear stage, rapid sample clean-up and precise positioning were achieved. Optimisation of spray solvent and nozzle placement improved performance, yielding high sensitivity, repeatability, linearity, and trueness with internal standard correction. This approach offers a simple, efficient solution for analysing organic residues in biological samples, with strong potential for future on-site applications.

AB - A novel method combining electroless ionisation mass spectrometry (ELI-MS) with solid-phase extraction (SPE) cartridges enables direct analysis of beta-agonist residues in bovine urine (SPE-ELI-MS). Using a syringe plunger and a battery-powered DIY syringe pump mounted on an XYZ-linear stage, rapid sample clean-up and precise positioning were achieved. Optimisation of spray solvent and nozzle placement improved performance, yielding high sensitivity, repeatability, linearity, and trueness with internal standard correction. This approach offers a simple, efficient solution for analysing organic residues in biological samples, with strong potential for future on-site applications.

KW - Ambient ionisation mass spectrometry

KW - Beta-agonists

KW - Bovine urine

KW - Electroless ionisation

KW - ELI-MS

KW - Solid-phase extraction

U2 - 10.1007/s00216-025-06019-3

DO - 10.1007/s00216-025-06019-3

M3 - Article

AN - SCOPUS:105011738195

SN - 1618-2642

VL - 417

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JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 22

ER -

Combining electroless ionisation mass spectrometry with solid-phase extraction for the direct analysis of beta-agonists in bovine urine

Abstract

Keywords

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Datasets

Combining electroless ionisation mass spectrometry with solid-phase extraction for the direct analysis of beta-agonists in bovine urine

Projects

Speerpunt 2 - Detecteren (KB-56-002-007, KB-37-002-037)

Cite this