Combining colloid milling and twin screw pressing for oleosome extraction

Oleosomes are oil droplets abundant in oilseeds that can be extracted together with proteins for use in emulsion-like foods and can be used as natural, ready emulsions as alternatives to dairy and fat-based products. To make optimal use of the raw material, we look at simultaneous extraction of oleosomes and proteins from rapeseed. The combined approach of colloid milling and twin screw pressing resulted in a higher yield of oleosomes (70 % w/w) when compared to twin screw press alone (54 % w/w), which may be due to extensive cell wall disruption caused by colloid mill processing, as evidenced by SEM imaging. The protein yield remained constant irrespective of the degree of cell wall disruption (50 % w/w). In conclusion, combining the colloid mill and twin screw press is a straightforward way for the extraction of oleosome and proteins from rapeseeds for food application.