Combined transcriptome and metabolome analysis identifies defence responses in spider mite-infested pepper (Capsicum annuum)

Yuanyuan Zhang, Harro J. Bouwmeester, Iris F. Kappers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Plants regulate responses towards herbivory through fine-tuning of defence-related hormone production, expression of defence genes, and production of secondary metabolites. Jasmonic acid (JA) plays a key role in plant–herbivorous arthropod interactions. To understand how pepper (Capsicum annuum) responds to herbivory, leaf transcriptomes and metabolomes of two genotypes different in their susceptibility to spider mites were studied. Mites induced both JA and salicylic acid (SA) signalling. However, mite infestation and exogenous JA resulted in distinct transcriptome profiles. Compared with JA, mites induced fewer differentially expressed genes involved in metabolic processes (except for genes involved in the phenylpropanoid pathway) and lipid metabolic processes. Furthermore, pathogen-related defence responses including WRKY transcription factors were more strongly induced upon mite infestation, probably as a result of induced SA signalling. Untargeted analysis of secondary metabolites confirmed that JA treatment induced larger changes in metabolism than spider mite infestation, resulting in higher terpenoid and flavonoid production. The more resistant genotype exhibited a larger increase in endogenous JA and volatile and non-volatile secondary metabolites upon infestation, which could explain its stronger defence. Reasoning that in JA–SA antagonizing crosstalk, SA defences are prioritized over JA defences, we hypothesize that lack of SA-mediated repression of JA-induced defences could result in gain of resistance towards spider mites in pepper.
Original languageEnglish
Pages (from-to)330-343
Number of pages14
JournalJournal of Experimental Botany
Volume71
Issue number1
Early online date26 Sep 2019
DOIs
Publication statusPublished - 1 Jan 2020

Fingerprint

Tetranychidae
Capsicum
Metabolome
metabolomics
Gene Expression Profiling
jasmonic acid
Capsicum annuum
transcriptomics
pepper
Salicylic Acid
Mite Infestations
mite infestations
salicylic acid
secondary metabolites
Herbivory
Mites
Transcriptome
transcriptome
mites
herbivores

Cite this

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title = "Combined transcriptome and metabolome analysis identifies defence responses in spider mite-infested pepper (Capsicum annuum)",
abstract = "Plants regulate responses towards herbivory through fine-tuning of defence-related hormone production, expression of defence genes, and production of secondary metabolites. Jasmonic acid (JA) plays a key role in plant–herbivorous arthropod interactions. To understand how pepper (Capsicum annuum) responds to herbivory, leaf transcriptomes and metabolomes of two genotypes different in their susceptibility to spider mites were studied. Mites induced both JA and salicylic acid (SA) signalling. However, mite infestation and exogenous JA resulted in distinct transcriptome profiles. Compared with JA, mites induced fewer differentially expressed genes involved in metabolic processes (except for genes involved in the phenylpropanoid pathway) and lipid metabolic processes. Furthermore, pathogen-related defence responses including WRKY transcription factors were more strongly induced upon mite infestation, probably as a result of induced SA signalling. Untargeted analysis of secondary metabolites confirmed that JA treatment induced larger changes in metabolism than spider mite infestation, resulting in higher terpenoid and flavonoid production. The more resistant genotype exhibited a larger increase in endogenous JA and volatile and non-volatile secondary metabolites upon infestation, which could explain its stronger defence. Reasoning that in JA–SA antagonizing crosstalk, SA defences are prioritized over JA defences, we hypothesize that lack of SA-mediated repression of JA-induced defences could result in gain of resistance towards spider mites in pepper.",
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Combined transcriptome and metabolome analysis identifies defence responses in spider mite-infested pepper (Capsicum annuum). / Zhang, Yuanyuan; Bouwmeester, Harro J.; Kappers, Iris F.

In: Journal of Experimental Botany, Vol. 71, No. 1, 01.01.2020, p. 330-343.

Research output: Contribution to journalArticleAcademicpeer-review

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