Combined normal-phase and reversed-phase liquid chromatography/ESI-MS as a tool to determine the molecular diversity of A-type procyanidins in peanut skins

M.M. Appeldoorn, J.P. Vincken, M.B. Sanders, P.C.H. Hollman, H. Gruppen

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)

Abstract

Peanut skins, a byproduct of the peanut butter industry, are a rich source of proanthocyanidins, which might be used in food supplements. Data on the molecular diversity of proanthocyanidins in peanut skins is limited and conflicting with respect to the ratio of double- (A-type) versus single-linked (B-type) flavan-3-ol units. NP- and RP-HPLC-MS were used as tools to analyze the molecular diversity of proanthocyanidins in a 20% (v/v) methanol extract of peanut skins. NP-HPLC was used to prepurify monomeric to pentameric fractions, which were further separated and characterized by RP-HPLC-MS. With this method, 83 different proanthocyanidin molecular species were characterized and quantified. Furthermore, it was possible to determine that A-type procyanidin oligomers were predominant and represented 95.0% (w/w) of the extract. In addition, the position of the A-linkages in 16 trimers and 27 tetramers could be determined, which in this case appeared to occur at all possible positions. The majority of trimers and tetramers with one or more A-linkage always had an A-linkage at the terminal unit
Original languageEnglish
Pages (from-to)6007-6013
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number14
DOIs
Publication statusPublished - 2009

Keywords

  • mass-spectrometry
  • proanthocyanidins
  • foods
  • oligomers
  • tannins

Fingerprint Dive into the research topics of 'Combined normal-phase and reversed-phase liquid chromatography/ESI-MS as a tool to determine the molecular diversity of A-type procyanidins in peanut skins'. Together they form a unique fingerprint.

Cite this