Collinearity between potato (Solanum tuberosum L.) and wild relatives assessed by comparative cytogenetic mapping

Paola Gaiero Guadagina, José Van De Belt, Francisco Vilaró, M.E. Schranz, Pablo Speranza, Hans de Jong*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

A major bottleneck to introgressive hybridization is the lack of genome collinearity between the donor (alien) genome and the recipient crop genome. Structural differences between the homeologs may create unbalanced segregation of chromosomes or cause linkage drag. To assess large-scale collinearity between potato and two of its wild relatives (Solanum commersonii and Solanum chacoense), we used BAC-FISH mapping of sequences with known positions on the RH potato map. BAC probes could successfully be hybridized to the S. commersonii and S. chachoense pachytene chromosomes, confirming their correspondence with linkage groups in RH potato. Our study shows that the order of BAC signals is conserved. Distances between BAC signals were quantified and compared; some differences found suggest either small-scale rearrangements or reduction/amplification of repeats. We conclude that S. commersonii and S. chacoense are collinear with cultivated Solanum tuberosum on the whole chromosome scale, making these amenable species for efficient introgressive hybridization breeding.
Original languageEnglish
Pages (from-to)228-240
JournalGenome
Volume60
Issue number3
DOIs
Publication statusPublished - 2017

Keywords

  • Introgressive hybridisation
  • Macrosynteny
  • Multicolour BAC-FISH
  • Potato
  • Solanum chacoense
  • Solanum commersonii

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