Abstract
Three different primary rat hepatocyte culture
methods were compared for their ability to allow the
secretion of fibrinogen and albumin under basal and IL-6-
stimulated conditions. These culture methods comprised the
co-culture of hepatocytes with rat liver epithelial cells (CCRLEC),
a collagen type I sandwich culture (SW) and a
conventional primary hepatocyte monolayer culture (ML).
Basal albumin secretion was most stable over time in SW.
Fibrinogen secretion was induced by IL-6 in all cell culture
models. Compared with ML, CC-RLEC showed an almost
three-fold higher fibrinogen secretion under both control
and IL-6-stimulated conditions. Induction of fibrinogen
release by IL-6 was lowest in SW. Albumin secretion was
decreased after IL-6 stimulation in both ML and CC-RLEC.
Thus, cells growing under the various primary hepatocyte
cell culture techniques react differently to IL-6 stimulation
with regard to acute-phase protein secretion. CC-RLEC is
the preferred method for studying cytokine-mediated
induction of acute-phase proteins, because of the pronounced
stimulation of fibrinogen secretion upon IL-6
exposure under these conditions.
Original language | English |
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Pages (from-to) | 451-457 |
Journal | Cell and Tissue Research |
Volume | 340 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2010 |
Keywords
- adult human hepatocytes
- long-term culture
- reference distributions
- hepatoma-cells
- collagen-gel
- large cohort
- in-vitro
- inflammation
- albumin
- serum