Co-culture of a novel fermentative bacterium, lucifera butyrica gen. Nov. Sp. Nov., With the sulfur reducer desulfurella amilsii for enhanced sulfidogenesis

Irene Sánchez-Andrea, Anna Patrícya Florentino, Jeltzlin Semerel, Nikolaos Strepis, Diana Z. Sousa, Alfons J.M. Stams

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Biosulfidogenesis can be used to remediate low pH and high metal content waters such as acid mine drainage and recover the present metals. The selection of a cheap electron donor for the process is important for the economic viability. In this work we isolated a novel versatile acidotolerant fermentative bacterium (strain ALET) that is able to use a great variety of substrates including glycerol. Strain ALET is an obligate anaerobe, and cells are motile, rod-shaped, spore-forming, and stain Gram-positive. Growth occurred in a pH range from 3.5 to 7 (optimum 5.5), and temperature range from 25 to 40∘C (optimum 37∘C). It grows by fermentation of sugars, organic acids and glycerol. It has the ability to use thiosulfate, iron and DMSO as electron acceptors. Its genome is 4.7 Mb with 5122 protein-coding sequences, and a G+C content of 46.9 mol%. Based on 16S rRNA gene sequence analysis, the closest cultured species is Propionispora hippei (91.4% 16S rRNA gene identity) from the Sporomusaceae family (Selenomonadales order, Negativicutes class, Firmicutes phylum). Based on the distinctive physiological and phylogenetic characteristics of strain ALET, a new genus and species Lucifera butyrica gen. nov., sp. nov., is proposed. The type strain is ALET (=JCM 19373T = DSM 27520T). Strain ALET is an incomplete oxidizer and acetate, among other products, accumulates during glycerol conversion. Strain ALET was used to extend the substrate range for sulfur reduction by constructing co-cultures with the acetate oxidizer and sulfur reducer Desulfurella amilsii. The co-culture was tested with glycerol as substrate in batch and chemostat experiments. Acetate formed by fermentation of glycerol by strain ALET resulted in sulfur reduction by D. amilsii. The co-culture strategy offers good perspectives to use a wide range of cost-efficient substrates, including glycerol, to produce sulfide by specialized sulfur reducers. The recovery of heavy metals from metalliferous streams may become economically feasible by this approach.

Original languageEnglish
Article number03108
JournalFrontiers in Microbiology
Volume9
DOIs
Publication statusPublished - 13 Dec 2018

Fingerprint

Coculture Techniques
Sulfur
Glycerol
Bacteria
Acetates
rRNA Genes
Fermentation
Metals
Electrons
Sugar Acids
Thiosulfates
Base Composition
Sulfides
Heavy Metals
Dimethyl Sulfoxide
Spores
Sequence Analysis
Drainage
Iron
Economics

Keywords

  • 1,3-PDO
  • Acidophilic sulfur reduction
  • Co-culture
  • Desulfurella amilsii
  • Glycerol
  • Lucifera butyrica

Cite this

@article{46b589ada4bc4d46b96ac4323ed52073,
title = "Co-culture of a novel fermentative bacterium, lucifera butyrica gen. Nov. Sp. Nov., With the sulfur reducer desulfurella amilsii for enhanced sulfidogenesis",
abstract = "Biosulfidogenesis can be used to remediate low pH and high metal content waters such as acid mine drainage and recover the present metals. The selection of a cheap electron donor for the process is important for the economic viability. In this work we isolated a novel versatile acidotolerant fermentative bacterium (strain ALET) that is able to use a great variety of substrates including glycerol. Strain ALET is an obligate anaerobe, and cells are motile, rod-shaped, spore-forming, and stain Gram-positive. Growth occurred in a pH range from 3.5 to 7 (optimum 5.5), and temperature range from 25 to 40∘C (optimum 37∘C). It grows by fermentation of sugars, organic acids and glycerol. It has the ability to use thiosulfate, iron and DMSO as electron acceptors. Its genome is 4.7 Mb with 5122 protein-coding sequences, and a G+C content of 46.9 mol{\%}. Based on 16S rRNA gene sequence analysis, the closest cultured species is Propionispora hippei (91.4{\%} 16S rRNA gene identity) from the Sporomusaceae family (Selenomonadales order, Negativicutes class, Firmicutes phylum). Based on the distinctive physiological and phylogenetic characteristics of strain ALET, a new genus and species Lucifera butyrica gen. nov., sp. nov., is proposed. The type strain is ALET (=JCM 19373T = DSM 27520T). Strain ALET is an incomplete oxidizer and acetate, among other products, accumulates during glycerol conversion. Strain ALET was used to extend the substrate range for sulfur reduction by constructing co-cultures with the acetate oxidizer and sulfur reducer Desulfurella amilsii. The co-culture was tested with glycerol as substrate in batch and chemostat experiments. Acetate formed by fermentation of glycerol by strain ALET resulted in sulfur reduction by D. amilsii. The co-culture strategy offers good perspectives to use a wide range of cost-efficient substrates, including glycerol, to produce sulfide by specialized sulfur reducers. The recovery of heavy metals from metalliferous streams may become economically feasible by this approach.",
keywords = "1,3-PDO, Acidophilic sulfur reduction, Co-culture, Desulfurella amilsii, Glycerol, Lucifera butyrica",
author = "Irene S{\'a}nchez-Andrea and Florentino, {Anna Patr{\'i}cya} and Jeltzlin Semerel and Nikolaos Strepis and Sousa, {Diana Z.} and Stams, {Alfons J.M.}",
year = "2018",
month = "12",
day = "13",
doi = "10.3389/fmicb.2018.03108",
language = "English",
volume = "9",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers",

}

Co-culture of a novel fermentative bacterium, lucifera butyrica gen. Nov. Sp. Nov., With the sulfur reducer desulfurella amilsii for enhanced sulfidogenesis. / Sánchez-Andrea, Irene; Florentino, Anna Patrícya; Semerel, Jeltzlin; Strepis, Nikolaos; Sousa, Diana Z.; Stams, Alfons J.M.

In: Frontiers in Microbiology, Vol. 9, 03108, 13.12.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Co-culture of a novel fermentative bacterium, lucifera butyrica gen. Nov. Sp. Nov., With the sulfur reducer desulfurella amilsii for enhanced sulfidogenesis

AU - Sánchez-Andrea, Irene

AU - Florentino, Anna Patrícya

AU - Semerel, Jeltzlin

AU - Strepis, Nikolaos

AU - Sousa, Diana Z.

AU - Stams, Alfons J.M.

PY - 2018/12/13

Y1 - 2018/12/13

N2 - Biosulfidogenesis can be used to remediate low pH and high metal content waters such as acid mine drainage and recover the present metals. The selection of a cheap electron donor for the process is important for the economic viability. In this work we isolated a novel versatile acidotolerant fermentative bacterium (strain ALET) that is able to use a great variety of substrates including glycerol. Strain ALET is an obligate anaerobe, and cells are motile, rod-shaped, spore-forming, and stain Gram-positive. Growth occurred in a pH range from 3.5 to 7 (optimum 5.5), and temperature range from 25 to 40∘C (optimum 37∘C). It grows by fermentation of sugars, organic acids and glycerol. It has the ability to use thiosulfate, iron and DMSO as electron acceptors. Its genome is 4.7 Mb with 5122 protein-coding sequences, and a G+C content of 46.9 mol%. Based on 16S rRNA gene sequence analysis, the closest cultured species is Propionispora hippei (91.4% 16S rRNA gene identity) from the Sporomusaceae family (Selenomonadales order, Negativicutes class, Firmicutes phylum). Based on the distinctive physiological and phylogenetic characteristics of strain ALET, a new genus and species Lucifera butyrica gen. nov., sp. nov., is proposed. The type strain is ALET (=JCM 19373T = DSM 27520T). Strain ALET is an incomplete oxidizer and acetate, among other products, accumulates during glycerol conversion. Strain ALET was used to extend the substrate range for sulfur reduction by constructing co-cultures with the acetate oxidizer and sulfur reducer Desulfurella amilsii. The co-culture was tested with glycerol as substrate in batch and chemostat experiments. Acetate formed by fermentation of glycerol by strain ALET resulted in sulfur reduction by D. amilsii. The co-culture strategy offers good perspectives to use a wide range of cost-efficient substrates, including glycerol, to produce sulfide by specialized sulfur reducers. The recovery of heavy metals from metalliferous streams may become economically feasible by this approach.

AB - Biosulfidogenesis can be used to remediate low pH and high metal content waters such as acid mine drainage and recover the present metals. The selection of a cheap electron donor for the process is important for the economic viability. In this work we isolated a novel versatile acidotolerant fermentative bacterium (strain ALET) that is able to use a great variety of substrates including glycerol. Strain ALET is an obligate anaerobe, and cells are motile, rod-shaped, spore-forming, and stain Gram-positive. Growth occurred in a pH range from 3.5 to 7 (optimum 5.5), and temperature range from 25 to 40∘C (optimum 37∘C). It grows by fermentation of sugars, organic acids and glycerol. It has the ability to use thiosulfate, iron and DMSO as electron acceptors. Its genome is 4.7 Mb with 5122 protein-coding sequences, and a G+C content of 46.9 mol%. Based on 16S rRNA gene sequence analysis, the closest cultured species is Propionispora hippei (91.4% 16S rRNA gene identity) from the Sporomusaceae family (Selenomonadales order, Negativicutes class, Firmicutes phylum). Based on the distinctive physiological and phylogenetic characteristics of strain ALET, a new genus and species Lucifera butyrica gen. nov., sp. nov., is proposed. The type strain is ALET (=JCM 19373T = DSM 27520T). Strain ALET is an incomplete oxidizer and acetate, among other products, accumulates during glycerol conversion. Strain ALET was used to extend the substrate range for sulfur reduction by constructing co-cultures with the acetate oxidizer and sulfur reducer Desulfurella amilsii. The co-culture was tested with glycerol as substrate in batch and chemostat experiments. Acetate formed by fermentation of glycerol by strain ALET resulted in sulfur reduction by D. amilsii. The co-culture strategy offers good perspectives to use a wide range of cost-efficient substrates, including glycerol, to produce sulfide by specialized sulfur reducers. The recovery of heavy metals from metalliferous streams may become economically feasible by this approach.

KW - 1,3-PDO

KW - Acidophilic sulfur reduction

KW - Co-culture

KW - Desulfurella amilsii

KW - Glycerol

KW - Lucifera butyrica

U2 - 10.3389/fmicb.2018.03108

DO - 10.3389/fmicb.2018.03108

M3 - Article

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 03108

ER -