Clostridium difficile in Dutch animals: their presence characteristics and similarities with human isolates

M.G.J. Koene, D. Mevius, J.A. Wagenaar, C. Harmanus, M.P.M. Hensgens, A.M. Meetsma, F.F. Putirulan, M.A.P. van Bergen, E.J. Kuijper

Research output: Contribution to journalArticleAcademicpeer-review

92 Citations (Scopus)

Abstract

The presence and characteristics of Clostridium difficile were investigated in 839 faecal samples from seven different animal species in the Netherlands. The number of positive samples ranged from 3.4% (cattle) to 25.0% (dogs). Twenty-two different PCR ribotypes were identified. Among 96 isolates, 53% harboured toxin genes. All C. difficile isolates from pigs, cattle and poultry were toxinogenic, whereas the majority of isolates from pet animals consisted of non-toxinogenic PCR ribotypes 010 and 039. Ribotype 012 was most prevalent in cattle and ribotype 078 in pigs. No predominant ribotypes were present in horse and poultry samples. Overall, PCR ribotypes 012, 014 and 078 were the most frequently recovered toxinogenic ribotypes from animal samples. Comparison with human isolates from the Dutch Reference Laboratory for C. difficile at Leiden University Medical Centre (LUMC) showed that these types were also recovered from human hospitalized patients in 2009/2010, encompassing 0.8%, 11.4% and 9.8% of all isolates, respectively. Application of multiple-locus variable-number tandem-repeat analysis indicated a genotypic relation of animal and human ribotype 078 strains, but a clear genotypic distinction for ribotypes 012 and 014. We conclude that toxinogenic C. difficile PCR ribotypes found in animals correspond to PCR ribotypes associated with human disease in hospitalized patients in the Netherlands. Contrary to PCR ribotype 078, significant genetic differences were observed between animal and human PCR ribotype 012 and 014 isolates
Original languageEnglish
Pages (from-to)778-784
JournalClinical Microbiology and Infection
Volume18
Issue number8
DOIs
Publication statusPublished - 2012

Fingerprint

Ribotyping
Clostridium difficile
Polymerase Chain Reaction
Poultry
Netherlands
Swine
Minisatellite Repeats
Pets
Horses

Keywords

  • tandem-repeat analysis
  • pcr ribotypes
  • disease
  • prevalence
  • infection
  • strains
  • calves
  • toxin
  • pigs

Cite this

Koene, M.G.J. ; Mevius, D. ; Wagenaar, J.A. ; Harmanus, C. ; Hensgens, M.P.M. ; Meetsma, A.M. ; Putirulan, F.F. ; van Bergen, M.A.P. ; Kuijper, E.J. / Clostridium difficile in Dutch animals: their presence characteristics and similarities with human isolates. In: Clinical Microbiology and Infection. 2012 ; Vol. 18, No. 8. pp. 778-784.
@article{55dc1da2e5cb48ecb1e2f66180a38233,
title = "Clostridium difficile in Dutch animals: their presence characteristics and similarities with human isolates",
abstract = "The presence and characteristics of Clostridium difficile were investigated in 839 faecal samples from seven different animal species in the Netherlands. The number of positive samples ranged from 3.4{\%} (cattle) to 25.0{\%} (dogs). Twenty-two different PCR ribotypes were identified. Among 96 isolates, 53{\%} harboured toxin genes. All C. difficile isolates from pigs, cattle and poultry were toxinogenic, whereas the majority of isolates from pet animals consisted of non-toxinogenic PCR ribotypes 010 and 039. Ribotype 012 was most prevalent in cattle and ribotype 078 in pigs. No predominant ribotypes were present in horse and poultry samples. Overall, PCR ribotypes 012, 014 and 078 were the most frequently recovered toxinogenic ribotypes from animal samples. Comparison with human isolates from the Dutch Reference Laboratory for C. difficile at Leiden University Medical Centre (LUMC) showed that these types were also recovered from human hospitalized patients in 2009/2010, encompassing 0.8{\%}, 11.4{\%} and 9.8{\%} of all isolates, respectively. Application of multiple-locus variable-number tandem-repeat analysis indicated a genotypic relation of animal and human ribotype 078 strains, but a clear genotypic distinction for ribotypes 012 and 014. We conclude that toxinogenic C. difficile PCR ribotypes found in animals correspond to PCR ribotypes associated with human disease in hospitalized patients in the Netherlands. Contrary to PCR ribotype 078, significant genetic differences were observed between animal and human PCR ribotype 012 and 014 isolates",
keywords = "tandem-repeat analysis, pcr ribotypes, disease, prevalence, infection, strains, calves, toxin, pigs",
author = "M.G.J. Koene and D. Mevius and J.A. Wagenaar and C. Harmanus and M.P.M. Hensgens and A.M. Meetsma and F.F. Putirulan and {van Bergen}, M.A.P. and E.J. Kuijper",
year = "2012",
doi = "10.1111/j.1469-0691.2011.03651.x",
language = "English",
volume = "18",
pages = "778--784",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Wiley",
number = "8",

}

Koene, MGJ, Mevius, D, Wagenaar, JA, Harmanus, C, Hensgens, MPM, Meetsma, AM, Putirulan, FF, van Bergen, MAP & Kuijper, EJ 2012, 'Clostridium difficile in Dutch animals: their presence characteristics and similarities with human isolates', Clinical Microbiology and Infection, vol. 18, no. 8, pp. 778-784. https://doi.org/10.1111/j.1469-0691.2011.03651.x

Clostridium difficile in Dutch animals: their presence characteristics and similarities with human isolates. / Koene, M.G.J.; Mevius, D.; Wagenaar, J.A.; Harmanus, C.; Hensgens, M.P.M.; Meetsma, A.M.; Putirulan, F.F.; van Bergen, M.A.P.; Kuijper, E.J.

In: Clinical Microbiology and Infection, Vol. 18, No. 8, 2012, p. 778-784.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Clostridium difficile in Dutch animals: their presence characteristics and similarities with human isolates

AU - Koene, M.G.J.

AU - Mevius, D.

AU - Wagenaar, J.A.

AU - Harmanus, C.

AU - Hensgens, M.P.M.

AU - Meetsma, A.M.

AU - Putirulan, F.F.

AU - van Bergen, M.A.P.

AU - Kuijper, E.J.

PY - 2012

Y1 - 2012

N2 - The presence and characteristics of Clostridium difficile were investigated in 839 faecal samples from seven different animal species in the Netherlands. The number of positive samples ranged from 3.4% (cattle) to 25.0% (dogs). Twenty-two different PCR ribotypes were identified. Among 96 isolates, 53% harboured toxin genes. All C. difficile isolates from pigs, cattle and poultry were toxinogenic, whereas the majority of isolates from pet animals consisted of non-toxinogenic PCR ribotypes 010 and 039. Ribotype 012 was most prevalent in cattle and ribotype 078 in pigs. No predominant ribotypes were present in horse and poultry samples. Overall, PCR ribotypes 012, 014 and 078 were the most frequently recovered toxinogenic ribotypes from animal samples. Comparison with human isolates from the Dutch Reference Laboratory for C. difficile at Leiden University Medical Centre (LUMC) showed that these types were also recovered from human hospitalized patients in 2009/2010, encompassing 0.8%, 11.4% and 9.8% of all isolates, respectively. Application of multiple-locus variable-number tandem-repeat analysis indicated a genotypic relation of animal and human ribotype 078 strains, but a clear genotypic distinction for ribotypes 012 and 014. We conclude that toxinogenic C. difficile PCR ribotypes found in animals correspond to PCR ribotypes associated with human disease in hospitalized patients in the Netherlands. Contrary to PCR ribotype 078, significant genetic differences were observed between animal and human PCR ribotype 012 and 014 isolates

AB - The presence and characteristics of Clostridium difficile were investigated in 839 faecal samples from seven different animal species in the Netherlands. The number of positive samples ranged from 3.4% (cattle) to 25.0% (dogs). Twenty-two different PCR ribotypes were identified. Among 96 isolates, 53% harboured toxin genes. All C. difficile isolates from pigs, cattle and poultry were toxinogenic, whereas the majority of isolates from pet animals consisted of non-toxinogenic PCR ribotypes 010 and 039. Ribotype 012 was most prevalent in cattle and ribotype 078 in pigs. No predominant ribotypes were present in horse and poultry samples. Overall, PCR ribotypes 012, 014 and 078 were the most frequently recovered toxinogenic ribotypes from animal samples. Comparison with human isolates from the Dutch Reference Laboratory for C. difficile at Leiden University Medical Centre (LUMC) showed that these types were also recovered from human hospitalized patients in 2009/2010, encompassing 0.8%, 11.4% and 9.8% of all isolates, respectively. Application of multiple-locus variable-number tandem-repeat analysis indicated a genotypic relation of animal and human ribotype 078 strains, but a clear genotypic distinction for ribotypes 012 and 014. We conclude that toxinogenic C. difficile PCR ribotypes found in animals correspond to PCR ribotypes associated with human disease in hospitalized patients in the Netherlands. Contrary to PCR ribotype 078, significant genetic differences were observed between animal and human PCR ribotype 012 and 014 isolates

KW - tandem-repeat analysis

KW - pcr ribotypes

KW - disease

KW - prevalence

KW - infection

KW - strains

KW - calves

KW - toxin

KW - pigs

U2 - 10.1111/j.1469-0691.2011.03651.x

DO - 10.1111/j.1469-0691.2011.03651.x

M3 - Article

VL - 18

SP - 778

EP - 784

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 8

ER -