Cloning of the chrysanthemum UEP1 promoter and comparative expression in leaves and ray and disc florets of Dendranthema grandiflora

S. Annadana, M.J. Beekwilder, G. Kuipers, P.B. Visser, N. Outchkourov, A. Pereira, M. Udayakumar, M.A. Jongsma

    Research output: Contribution to journalArticleAcademicpeer-review

    20 Citations (Scopus)

    Abstract

    To attain high transgene expression in petal tissue of ray florets of chrysanthemum an endogenous ubiquitin extension protein (UEP1) promoter was cloned and tested with the β-glucuronidase (GUS) reporter gene. Expression levels were compared with four heterologous promoters: chalcone synthase (chs-A) and zinc finger transcription factor (EPF2-5) from petunia, eceriferum (CER6) from Arabidopsis and multicystatin (PMC) from potato. The comparison of the expression levels of the different constructs in ray florets, disc florets, and leaves is presented. The highest mean expression in petal tissue of ray and disc florets was conferred by the UEP1 promoter, followed by CER6 and EPF2-5. The UEP1 promoter in ray florets confers over 50-fold enhancement in expression as compared to CaMV 35S-based promoters.
    Original languageEnglish
    Pages (from-to)437-445
    JournalTransgenic Research
    Volume11
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Chrysanthemum
    Chrysanthemum morifolium
    florets
    Organism Cloning
    molecular cloning
    promoter regions
    Petunia
    Glucuronidase
    Zinc Fingers
    Solanum tuberosum
    Ubiquitin
    Transgenes
    Reporter Genes
    Arabidopsis
    leaves
    Transcription Factors
    corolla
    naringenin-chalcone synthase
    zinc finger motif
    ubiquitin

    Cite this

    Annadana, S. ; Beekwilder, M.J. ; Kuipers, G. ; Visser, P.B. ; Outchkourov, N. ; Pereira, A. ; Udayakumar, M. ; Jongsma, M.A. / Cloning of the chrysanthemum UEP1 promoter and comparative expression in leaves and ray and disc florets of Dendranthema grandiflora. In: Transgenic Research. 2002 ; Vol. 11. pp. 437-445.
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    title = "Cloning of the chrysanthemum UEP1 promoter and comparative expression in leaves and ray and disc florets of Dendranthema grandiflora",
    abstract = "To attain high transgene expression in petal tissue of ray florets of chrysanthemum an endogenous ubiquitin extension protein (UEP1) promoter was cloned and tested with the β-glucuronidase (GUS) reporter gene. Expression levels were compared with four heterologous promoters: chalcone synthase (chs-A) and zinc finger transcription factor (EPF2-5) from petunia, eceriferum (CER6) from Arabidopsis and multicystatin (PMC) from potato. The comparison of the expression levels of the different constructs in ray florets, disc florets, and leaves is presented. The highest mean expression in petal tissue of ray and disc florets was conferred by the UEP1 promoter, followed by CER6 and EPF2-5. The UEP1 promoter in ray florets confers over 50-fold enhancement in expression as compared to CaMV 35S-based promoters.",
    author = "S. Annadana and M.J. Beekwilder and G. Kuipers and P.B. Visser and N. Outchkourov and A. Pereira and M. Udayakumar and M.A. Jongsma",
    year = "2002",
    doi = "10.1023/A:1016313924844",
    language = "English",
    volume = "11",
    pages = "437--445",
    journal = "Transgenic Research",
    issn = "0962-8819",
    publisher = "Springer Verlag",

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    Cloning of the chrysanthemum UEP1 promoter and comparative expression in leaves and ray and disc florets of Dendranthema grandiflora. / Annadana, S.; Beekwilder, M.J.; Kuipers, G.; Visser, P.B.; Outchkourov, N.; Pereira, A.; Udayakumar, M.; Jongsma, M.A.

    In: Transgenic Research, Vol. 11, 2002, p. 437-445.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Cloning of the chrysanthemum UEP1 promoter and comparative expression in leaves and ray and disc florets of Dendranthema grandiflora

    AU - Annadana, S.

    AU - Beekwilder, M.J.

    AU - Kuipers, G.

    AU - Visser, P.B.

    AU - Outchkourov, N.

    AU - Pereira, A.

    AU - Udayakumar, M.

    AU - Jongsma, M.A.

    PY - 2002

    Y1 - 2002

    N2 - To attain high transgene expression in petal tissue of ray florets of chrysanthemum an endogenous ubiquitin extension protein (UEP1) promoter was cloned and tested with the β-glucuronidase (GUS) reporter gene. Expression levels were compared with four heterologous promoters: chalcone synthase (chs-A) and zinc finger transcription factor (EPF2-5) from petunia, eceriferum (CER6) from Arabidopsis and multicystatin (PMC) from potato. The comparison of the expression levels of the different constructs in ray florets, disc florets, and leaves is presented. The highest mean expression in petal tissue of ray and disc florets was conferred by the UEP1 promoter, followed by CER6 and EPF2-5. The UEP1 promoter in ray florets confers over 50-fold enhancement in expression as compared to CaMV 35S-based promoters.

    AB - To attain high transgene expression in petal tissue of ray florets of chrysanthemum an endogenous ubiquitin extension protein (UEP1) promoter was cloned and tested with the β-glucuronidase (GUS) reporter gene. Expression levels were compared with four heterologous promoters: chalcone synthase (chs-A) and zinc finger transcription factor (EPF2-5) from petunia, eceriferum (CER6) from Arabidopsis and multicystatin (PMC) from potato. The comparison of the expression levels of the different constructs in ray florets, disc florets, and leaves is presented. The highest mean expression in petal tissue of ray and disc florets was conferred by the UEP1 promoter, followed by CER6 and EPF2-5. The UEP1 promoter in ray florets confers over 50-fold enhancement in expression as compared to CaMV 35S-based promoters.

    U2 - 10.1023/A:1016313924844

    DO - 10.1023/A:1016313924844

    M3 - Article

    VL - 11

    SP - 437

    EP - 445

    JO - Transgenic Research

    JF - Transgenic Research

    SN - 0962-8819

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