Cloning of a prolidase gene from Aspergillus nidulans and characterisation of its product

R. Jalving, P. Bron, H.C.M. Kester, J. Visser, P.J. Schaap

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

Using EST sequence information available from the filamentous fungus Aspergillus nidulans as a starting point, we have cloned the prolidase-encoding gene, designated pepP. Introduction of multiple copies of this gene into the A. nidulans genome leads to overexpression of an intracellular prolidase activity. Prolidase was subsequently purified and characterised from an overexpressing strain. The enzyme activity is dependent on manganese as a cofactor, is specific for dipeptides and hydrolyses only dipeptides with a C-terminal proline residue. Although these proline dipeptides are released both intracellularly and extracellularly, prolidase activity was detected only intracellularly
Original languageEnglish
Pages (from-to)218-222
JournalMolecular Genetics and Genomics
Volume267
Issue number2
DOIs
Publication statusPublished - 2002

    Fingerprint

Keywords

  • escherichia-coli
  • transformation
  • aminopeptidase
  • purification
  • expression
  • peptides

Cite this