Cloning, characterization and heterologous expression of epoxide hydrolase-encoding cDNA sequences from yeasts belonging to the genera Rhodotorula and Rhodosporidium

H. Visser; C.A.G.M. Weijers; A.J.J. van Ooyen; J.C. Verdoes

doi:10.1023/A:1020613803342

Cloning, characterization and heterologous expression of epoxide hydrolase-encoding cDNA sequences from yeasts belonging to the genera Rhodotorula and Rhodosporidium

H. Visser, C.A.G.M. Weijers, A.J.J. van Ooyen, J.C. Verdoes

Research output: Contribution to journal › Article › Academic › peer-review

18 Citations (Scopus)

Abstract

Epoxide hydrolase-encoding cDNA sequences were isolated from the basidiomycetous yeast species Rhodosporidium toruloides CBS 349, Rhodosporidium toruloides CBS 14 and Rhodotorula araucariae CBS 6031 in order to evaluate the molecular data and potential application of this type of enzymes. The deduced amino acid sequences were similar to those of the known epoxide hydrolases from Rhodotorula glutinis CBS 8761, Xanthophyllomyces dendrorhous CBS 6938 and Aspergillus niger LCP 521, which all correspond to the group of the microsomal epoxide hydrolases. The epoxide hydrolase encoding cDNAs of the Rhodosporidium and Rhodotorula species were expressed in Escherichia coli. The recombinant strains were able to hydrolyze trans-1-phenyl-1,2-epoxypropane with high enantioselectivity.

Original language	English
Pages (from-to)	1687-1694
Journal	Biotechnology Letters
Volume	24
DOIs	https://doi.org/10.1023/A:1020613803342
Publication status	Published - 2002

Keywords

cDNA
Epoxide hydrolase
Functional expression
Rhodosporidium
Rhodotorula

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10.1023/A:1020613803342

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title = "Cloning, characterization and heterologous expression of epoxide hydrolase-encoding cDNA sequences from yeasts belonging to the genera Rhodotorula and Rhodosporidium",

abstract = "Epoxide hydrolase-encoding cDNA sequences were isolated from the basidiomycetous yeast species Rhodosporidium toruloides CBS 349, Rhodosporidium toruloides CBS 14 and Rhodotorula araucariae CBS 6031 in order to evaluate the molecular data and potential application of this type of enzymes. The deduced amino acid sequences were similar to those of the known epoxide hydrolases from Rhodotorula glutinis CBS 8761, Xanthophyllomyces dendrorhous CBS 6938 and Aspergillus niger LCP 521, which all correspond to the group of the microsomal epoxide hydrolases. The epoxide hydrolase encoding cDNAs of the Rhodosporidium and Rhodotorula species were expressed in Escherichia coli. The recombinant strains were able to hydrolyze trans-1-phenyl-1,2-epoxypropane with high enantioselectivity.",

keywords = "cDNA, Epoxide hydrolase, Functional expression, Rhodosporidium, Rhodotorula",

author = "H. Visser and C.A.G.M. Weijers and {van Ooyen}, A.J.J. and J.C. Verdoes",

year = "2002",

doi = "10.1023/A:1020613803342",

language = "English",

volume = "24",

pages = "1687--1694",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer",

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TY - JOUR

T1 - Cloning, characterization and heterologous expression of epoxide hydrolase-encoding cDNA sequences from yeasts belonging to the genera Rhodotorula and Rhodosporidium

AU - Visser, H.

AU - Weijers, C.A.G.M.

AU - van Ooyen, A.J.J.

AU - Verdoes, J.C.

PY - 2002

Y1 - 2002

N2 - Epoxide hydrolase-encoding cDNA sequences were isolated from the basidiomycetous yeast species Rhodosporidium toruloides CBS 349, Rhodosporidium toruloides CBS 14 and Rhodotorula araucariae CBS 6031 in order to evaluate the molecular data and potential application of this type of enzymes. The deduced amino acid sequences were similar to those of the known epoxide hydrolases from Rhodotorula glutinis CBS 8761, Xanthophyllomyces dendrorhous CBS 6938 and Aspergillus niger LCP 521, which all correspond to the group of the microsomal epoxide hydrolases. The epoxide hydrolase encoding cDNAs of the Rhodosporidium and Rhodotorula species were expressed in Escherichia coli. The recombinant strains were able to hydrolyze trans-1-phenyl-1,2-epoxypropane with high enantioselectivity.

AB - Epoxide hydrolase-encoding cDNA sequences were isolated from the basidiomycetous yeast species Rhodosporidium toruloides CBS 349, Rhodosporidium toruloides CBS 14 and Rhodotorula araucariae CBS 6031 in order to evaluate the molecular data and potential application of this type of enzymes. The deduced amino acid sequences were similar to those of the known epoxide hydrolases from Rhodotorula glutinis CBS 8761, Xanthophyllomyces dendrorhous CBS 6938 and Aspergillus niger LCP 521, which all correspond to the group of the microsomal epoxide hydrolases. The epoxide hydrolase encoding cDNAs of the Rhodosporidium and Rhodotorula species were expressed in Escherichia coli. The recombinant strains were able to hydrolyze trans-1-phenyl-1,2-epoxypropane with high enantioselectivity.

KW - cDNA

KW - Epoxide hydrolase

KW - Functional expression

KW - Rhodosporidium

KW - Rhodotorula

U2 - 10.1023/A:1020613803342

DO - 10.1023/A:1020613803342

M3 - Article

SN - 0141-5492

VL - 24

SP - 1687

EP - 1694

JO - Biotechnology Letters

JF - Biotechnology Letters

ER -

Cloning, characterization and heterologous expression of epoxide hydrolase-encoding cDNA sequences from yeasts belonging to the genera Rhodotorula and Rhodosporidium

Abstract

Keywords

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