Cloning and Functional Analysis of three Cold Regulated CBF Genes in the Overwintering Crucifer Boechera stricta

Jaeyun Heo*, Peter van Tienderen, M.E. Schranz

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

In this research, we isolated three CBF (C-repeat-Binding Factors) genes from two genotypes of Boechera stricta with contrasting freezing tolerance and characterized their structure and expression patterns in response to cold treatment. An amino acid sequence comparison revealed that the CBF genes in B. stricta showed high conservation in the AP2 domain and PKKP/RAGR motif like other cold adaptable Brassicaceae. The pairwise sequence alignment of the CBF genes isolated from two genotypes of B. stricta showed non-synonymous mutations in CBF 2 and 3. Gene expression analysis demonstrated that
CBF genes in B. stricta have expression patterns similar to CBFs in A. thaliana in response to cold treatment, while differential expression at the molecular level in CBF and COR genes was presented between two genotypes of B. stricta. Our
results suggest that signal transduction of three CBF genes can be one of the central pathways in the development of freezing tolerance in B. stricta.
Original languageEnglish
Pages (from-to)594-600
JournalInternational Journal of Agriculture & Biology
Volume20
Issue number3
DOIs
Publication statusPublished - 2018

Fingerprint Dive into the research topics of 'Cloning and Functional Analysis of three Cold Regulated CBF Genes in the Overwintering Crucifer Boechera stricta'. Together they form a unique fingerprint.

  • Cite this