Cloning and functional analyses of a gene from sugar beet up-regulated upon cyst nematode infection

S. Samuelian, M. Kleine, C.P. Spira, R.M. Klein Lankhorst, C. Jung

    Research output: Contribution to journalArticleAcademicpeer-review

    13 Citations (Scopus)

    Abstract

    The cDNA-AFLP technique was used to isolate sugar beet genes up-regulated upon infection with the beet cyst nematode Heterodera schachtii. Hairy root cultures were obtained from resistant plants carrying a Beta procumbens translocation as well as from a non-resistant control. mRNA was isolated from hairy root clones and sugar beet plants infected or not with the beet cyst nematode and 8000 transcript-derived fragments (TDFs) were analysed. One TDF was found to be differentially expressed in both materials and was further investigated. Real-time PCR confirmed that this TDF is specifically up-regulated in resistant sugar beet upon nematode infection and its full-length cDNA was isolated. Sequence analysis suggests that the gene encodes a 317 amino acid polypeptide of unknown function. No homology to any sequence present in the public databases could be detected. To further elucidate its function in resistance to the beet cyst nematode, the cDNA was transformed into hairy roots of susceptible sugar beet under the control of the 35S promoter and hairy root clones were inoculated with nematodes. The number of developing females was significantly reduced in 12 out of 15 clones resulting from independent transgenic events suggesting that the gene can be used for inducing cyst nematode resistance in plants
    Original languageEnglish
    Pages (from-to)147-156
    JournalPlant Molecular Biology
    Volume54
    Issue number1
    DOIs
    Publication statusPublished - 2004

    Keywords

    • transgenic hairy roots
    • host-cell responses
    • arabidopsis-thaliana
    • confers resistance
    • disease resistance
    • defense responses
    • knot nematode
    • expression
    • aflp
    • plants

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